Sleep is a profound regulator of cellular immunity, and the curtailment of sleep in present day lifestyle leads to disruption of neuro-immune-endocrine interactions. No therapeutic remedy is yet known for the amelioration of detrimental effects caused by sleep deprivation (SD). The current study was aimed to elucidate the effects of acute SD on immune function and its modulation by water extract from leaves of Withania somnifera (ASH-WEX). Three groups of animals, i.e. Vehicle-Undisturbed sleep (VUD), Vehicle-Sleep deprived (VSD) and ASH-WEX fed sleep deprived (WSD) rats were tested for their anxiety-like behaviour and further used for the study of inflammatory and apoptotic markers expression in piriform cortex and hippocampus regions of the brain. VSD animals showed high level of anxiety in elevated plus maze test, which was ameliorated in WSD group. The stress induced expression of inflammatory and immune response markers GFAP, TNFα, IL-6, OX-18 and OX-42 in VSD animals was found to be modulated by ASH-WEX. Further, the stress induced apoptosis was suppressed in WSD group as indicated by expression of NF-κB, AP-1, Bcl-xL and Cytochrome c. This study provides scientific validation to the anxiolytic, anti-inflammatory and anti-apoptotic properties of ASH-WEX, which may serve as an effective dietary supplement for management of SD induced stress and associated functional impairments.

译文

睡眠是细胞免疫的深刻调节剂,当今生活方式中睡眠的减少导致神经-免疫-内分泌相互作用的破坏。尚无治疗方法可以改善由睡眠剥夺 (SD) 引起的有害影响。当前的研究旨在阐明急性SD对免疫功能的影响及其通过witsania somniifera (ASH-WEX) 叶片中的水提取物的调节作用。三组动物,即车辆-不受干扰的睡眠 (VUD),测试了载体睡眠剥夺 (VSD) 和ASH-WEX喂养的睡眠剥夺 (WSD) 大鼠的焦虑样行为,并进一步用于研究梨状皮层和大脑海马区的炎症和凋亡标志物表达。VSD动物在升高加迷宫试验中表现出较高的焦虑水平,这在WSD组中得到改善。发现在VSD动物中,应激诱导的炎症和免疫反应标记物GFAP,tnf α,IL-6,OX-18和OX-42的表达受到ASH-WEX的调节。此外,如NF-κ b,AP-1,Bcl-xL和细胞色素c的表达所示,WSD组应激诱导的细胞凋亡受到抑制。该研究为ASH-WEX的抗焦虑,抗炎和抗凋亡特性提供了科学验证。它可以作为有效的膳食补充剂,用于管理SD引起的压力和相关的功能障碍。

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