The relevance of 5-HT1A and 5-HT2C receptors of the basolateral nucleus of the amygdala (BLA) in the mediation of anxiety-related defensive responses has long been acknowledged. Whereas strong evidence supports that activation of the latter receptors provokes anxiety, conflicting findings have been reported on the role played by the former binding site. In this study we further investigated the involvement of 5-HT1A receptors (5-HT1A-Rs) in the regulation of anxiety- and panic-related defensive behaviors. The results showed that intra-BLA injection of the 5-HT1A-R agonist 8-OH-DPAT (0.4-16nmol) in male Wistar rats impaired the acquisition of inhibitory avoidance in the elevated T-maze, increased the percentage of time spent in the lit compartment of the light-dark transition model and enhanced the number of punished drinking events in the Vogel conflict test, all changes compatible with an anxiolytic effect. This agonist also impaired escape expression in the elevated T-maze, suggestive of a panicolytic-like effect. 8-OH-DPAT-induced changes in the elevated T-maze and light-dark tests were blocked by previous local administration of the 5-HT1A-R antagonist WAY-100635 (0.37nmol) and were also observed after intra-BLA microinjection of the benzodiazepine receptor agonist midazolam (10-40nmol). Thus, stimulation of 5-HT1A-Rs in the BLA causes both anxiolytic- and panicolytic-like effects, what may have implications for the pathophysiology and treatment of generalized anxiety and panic disorders.

译文

杏仁核 (BLA) 基底外侧核的5-HT1A和5-HT2C受体与焦虑相关的防御反应的介导之间的相关性早已得到认可。尽管有强有力的证据支持后者受体的激活引起焦虑,但据报道,前一个结合位点的作用存在矛盾。在这项研究中,我们进一步研究了5-HT1A受体 (5-HT1A-Rs) 参与焦虑和恐慌相关防御行为的调节。结果表明,在雄性Wistar大鼠中BLA内注射5-HT1A-R激动剂8-OH-DPAT (0.4-16nmol) 会损害T型迷宫中抑制性回避的获得,在Vogel冲突测试中,增加了在明暗过渡模型的照明室中花费的时间百分比,并增加了受惩罚的饮酒事件的数量,所有变化均与抗焦虑作用兼容。这种激动剂还损害了升高的T迷宫中的逃逸表达,暗示了类解毒作用。通过先前局部施用5-HT1A-R拮抗剂WAY-100635 (0.37nmol) 阻断了升高的T迷宫和明暗测试中的8-oh-dpat诱导的变化,并且在BLA内微注射苯二氮卓受体激动剂咪达唑仑 (10-40nmol) 后也观察到。因此,在BLA中刺激5-HT1A-Rs会引起抗焦虑和类抗焦虑作用,这可能对广泛性焦虑症和恐慌症的病理生理学和治疗产生影响。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录