Repetitive transcranial magnetic stimulation (rTMS) treatment is widely accepted as an evidence-based treatment option for depression and anxiety. However, the underlying mechanism of this treatment maneuver has not been clearly understood. The chronic unpredictable mild stress (CUMS) procedure was used to establish depression and anxiety-like behavior in rats. The rTMS was performed with a commercially available stimulator for seven consecutive days, and then depression and anxiety-like behaviors were subsequently measured. The expression of nuclear factor-E2-related factor 2 (Nrf2) was measured by western-blot, and the level of tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), and interleukin-6 (IL-6) was measured with Enzyme-linked immunesorbent assay (ELISA) analyzing kits. Furthermore, a small interfering RNA was employed to knockdown Nrf2, after which the neurobehavioral assessment, Nrf2 nuclear expression, and the amount of inflammation factors were evaluated. Application of rTMS exhibited a significant antidepressant and anxiolytic-like effect, which was associated with the increased Nrf2 nuclear translocation and reduced level of TNF-α, iNOS, IL-1β, and IL-6 in the hippocampus. Following Nrf2 silencing, the antidepressant and anxiolytic-like effect produced by rTMS was abolished. Moreover, the elevated Nrf2 nuclear translocation, and the reduced production of TNF-α, iNOS, IL-1β, and IL-6 in hippocampus mediated by rTMS, were reversed by Nrf2 knockdown. Together, these results reveal that the Nrf2-induced anti-inflammation effect is crucial in regulating antidepressant-related behaviors produced by rTMS.

译文

重复经颅磁刺激 (rTMS) 治疗被广泛接受为抑郁症和焦虑的循证治疗选择。但是,这种治疗方法的潜在机制尚未清楚。慢性不可预测的轻度应激 (CUMS) 程序用于建立大鼠的抑郁和焦虑样行为。连续7天使用市售刺激器进行rTMS,然后测量抑郁和焦虑样行为。蛋白质印迹法检测核factor-E2-related因子2 (Nrf2) 的表达,肿瘤坏死因子-α (TNF-α) 、诱导型一氧化氮合酶 (iNOS) 、白细胞介素-1β (IL-1β) 、用酶联免疫吸附测定 (ELISA) 分析试剂盒测量interleukin-6 (IL-6)。此外,使用小干扰RNA敲除Nrf2,然后评估神经行为评估,Nrf2核表达和炎症因子的量。rTMS的应用表现出明显的抗抑郁和抗焦虑样作用,这与Nrf2核易位增加以及海马中TNF-α,iNOS,IL-1β 和IL-6水平降低有关。Nrf2沉默后,rTMS产生的抗抑郁和抗焦虑作用被取消。此外,Nrf2敲低抑制逆转了由rTMS介导的海马中Nrf2核易位的升高以及TNF-α,iNOS,IL-1β 和IL-6的减少。总之,这些结果表明,Nrf2-induced的抗炎作用在调节由rTMS产生的抗抑郁药相关行为中至关重要。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录