Membrane rigidification could be the first step of cold perception in poikilotherms. We have investigated its implication in diacylglycerol kinase (DAGK) activation by cold stress in suspension cells from Arabidopsis mutants altered in desaturase activities. By lateral diffusion assay, we showed that plasma membrane rigidification with temperature decrease was steeper in cells deficient in oleate desaturase than in wild type cells and in cells overexpressing linoleate desaturase. The threshold for the activation of the DAGK pathway in each type of cells correlated with this order of rigidification rate, suggesting that cold induced-membrane rigidification is upstream of DAGK pathway activation.

译文

膜硬化可能是poikilotherms中冷知觉的第一步。我们已经研究了其在拟南芥突变体的悬浮细胞中冷胁迫对二酰基甘油激酶 (DAGK) 激活的影响去饱和酶活性改变。通过横向扩散试验,我们发现,缺乏油酸去饱和酶的细胞的质膜硬化比野生型细胞和过表达亚油酸去饱和酶的细胞更陡峭。每种类型细胞中DAGK途径激活的阈值与该硬化速率的顺序相关,这表明冷诱导的膜硬化是DAGK途径激活的上游。

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