Environmental endocrine disruptors 4-nonylphenol (NP) and 4-tert-octylphenol (OP) may cast huge harm to human health. We used a rat model to observe the influence of NP or/and OP exposure on anxiety-related behaviors and the underlying mechanisms. Eighty male Sprague-Dawley (SD) rats were randomly divided into 10 groups: control group (corn oil), NP groups [30, 90, 270 mg/kg], OP groups [40, 120, 360 mg/kg] and NO groups [(mixed with the corresponding NP, OP alone exposed low, medium and high dose according to the natural environment exists NP:OP = 4:1]. The rats were orally administered every other day for 30 days. The neurobehaviors of rats were evaluated by open-field test (OFT) and elevated plus-maze test (EPM), and the concentrations of 5-HT, monoamine oxidase (MAOA), serotonin transporter (SERT), vesicular monoamine transporter 2 (VAMT2), 5-hydroxytryptamine 1A (5-HT1A), 5-hydroxytryptamine 2A (5-HT2A),and 5-hydroxytryptamine 2C (5-HT2C) in the rat prefrontal cortex were analyzed by ELISA. OFT and EPM tests showed that NP or/and OP exposure induced anxiety-related behaviors in rats. 5-HT levels were significantly increased compared with the control group. The levels of MAOA, SERT, VAMT2, 5-HT1A, 5-HT2A, and 5-HT2C in the prefrontal cortex reduced in different degrees by high-doses NP or/and OP exposure. In summary, NP or/and OP exposure might cause anxiety-related behaviors in rats through regulating neurotransmitter 5-HT levels by altering the expression of 5-HT decomposition enzyme MAOA, transporters SERT and VMAT2, and 5-HT receptors 5-HT1A, 5-HT2A, and 5-HT2C.

译文

环境内分泌干扰物4-壬基酚 (NP) 和4-叔辛基酚 (OP) 可能对人体健康造成巨大危害。我们使用大鼠模型观察NP或/和OP暴露对焦虑相关行为和潜在机制的影响。80只雄性Sprague-Dawley (SD) 大鼠随机分为10组: 对照组 (玉米油),NP组 [30、90、270 mg/kg],OP组 [40、120、360 mg/kg] 和NO组 [(与相应的NP,根据自然环境单独暴露低、中、高剂量的OP存在NP:OP = 4:1]。大鼠每隔一天口服给药30天。大鼠的神经行为通过野外试验 (OFT) 和升高迷宫试验 (EPM) 进行评估,5-HT,单胺氧化酶 (MAOA),5-羟色胺转运蛋白 (SERT),囊泡单胺转运蛋白2 (VAMT2),5-羟色胺1A (5-HT1A),5-羟色胺2A (5-HT2A),和5-羟色胺2C (5-HT2C) 在大鼠前额叶皮层进行ELISA分析。OFT和EPM测试表明,NP或/和OP暴露诱导大鼠焦虑相关行为。5-HT水平与对照组相比明显升高。MAOA,SERT,VAMT2,5-HT1A,5-HT2A,高剂量NP或/和OP暴露使前额叶皮层的5-HT2C有不同程度的降低。总之,NP或/和OP暴露可能通过改变5-HT分解酶MAOA,转运蛋白SERT和VMAT2的表达来调节神经递质5-HT水平,从而引起大鼠的焦虑相关行为,和5-HT受体5-HT1A,5-HT2A和5-HT2C。

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