The phototransformation pathways of protochlorophyllide forms were studied in 8-14-day-old leaves of dark-germinated wheat (Triticum aestivum L.) using white, 632.8 nm He-Ne laser and 654 nm laser diode light. The photon flux density (PFD) values (0.75-360 μmol photons m(-2) s(-1)), the illumination periods (20 ms-10 s) and the temperature of the leaves (between -60 °C and room temperature) were varied. The 77 K fluorescence spectra of partially phototransformed leaves showed gradual accumulation or even the dominance of the 675 nm emitting chlorophyllide or chlorophyll form at room temperature with 632.8 nm of PFD less than 200 μmol photons m(-2) s(-1) or with 654 nm of low PFD (7.5 μmol photons m(-2) s(-1)) up to 1 s. Longer wavelength (685 or 690 nm) emitting chlorophyllide forms appeared at illuminations under -25 °C with both laser lights or at room temperature when the PFD values were higher or the illumination period was longer than above. We concluded that the formation of the 675 nm emitting chlorophyllide form does not indicate the direct photoactivity of the 633 nm emitting protochlorophyllide form; it can derive from 644 and 657 nm forms via instantaneous disaggregation of the newly-produced chlorophyllide complexes. The disaggregation is strongly influenced by the molecular environment and the localization of the complex.

译文

使用白色,632.8 nm He-Ne激光和654 nm激光二极管光,在8-14日龄的深色发芽小麦 (Triticum aestivum L.) 的叶片中研究了原叶绿素形式的光转化途径。光子通量密度 (PFD) 值 (0.75-360 μ mol光子m(-2) s(-1)),光照周期 (20 ms-10 s) 和叶片温度 (-60 °C和室温之间) 变化。部分光转化的叶片的77 k荧光光谱显示,在室温下,632.8 nm的PFD小于200 μ mol光子m(-2) s(-1) 或654 nm的低PFD (7.5 μ mol光子m(-2) s(-1)) 最多1 s。当PFD值较高或照射周期长于以上时,在-25 °C下用两种激光照射下或在室温下,发射叶绿素形式出现更长的波长 (685或690 nm)。我们得出的结论是,发射675 nm的叶绿素形式的形成并不表明发射633 nm的原叶绿素形式的直接光活性; 它可以通过新产生的叶绿素复合物的瞬时分解从644和657 nm形式衍生而来。分解受到分子环境和复合物定位的强烈影响。

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