A novel bovine interferon-ω (BoIFN-ω) gene, which encodes a protein of 195 amino acids with a 23-amino acid signal peptide, was amplified from bovine liver genomic DNA through PCR and named BoIFN-ω24 according to its position in the bovine genome. In this study, the recombinant protein was expressed in Escherichia coli and antiviral or antiproliferation activity was determined in vitro. Results showed that BoIFN-ω24 exhibits high antiviral activity, which can be abrogated using PAb against BoIFN-ω24, and inhibits cell proliferation. BoIFN-ω24 also presents high sensitivity to trypsin and stability at pH 2.0 or 65°C, which are typical characteristics of type I IFN. This study revealed that BoIFN-ω24 is a potential novel effective therapeutic agent and provided a basis for further research on the BoIFN-ω multigene family.

译文

通过PCR从牛肝基因组DNA中扩增出一个新的牛干扰素-ω (BoIFN-ω) 基因,该基因编码具有23个氨基酸信号肽的195个氨基酸的蛋白质,并根据其在牛基因组中的位置将其命名为BoIFN-ω24。在这项研究中,重组蛋白在大肠杆菌中表达,并在体外确定了抗病毒或抗增殖活性。结果表明,BoIFN-ω24具有很高的抗病毒活性,可以使用PAb消除BoIFN-ω24,并抑制细胞增殖。BoIFN-ω24还表现出对胰蛋白酶的高灵敏度和在pH 2.0或65 °C下的稳定性,这是I型IFN的典型特征。这项研究表明,BoIFN-ω24是一种潜在的新型有效治疗剂,并为进一步研究BoIFN-ω 多基因家族提供了基础。

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