Human rhinovirus (HRV), a single-stranded, positive-sense RNA virus, is associated with mild upper respiratory tract infections in children. The aim of this study was to carry out a molecular characterization and phylogeny-based classification of the circulating genotypes of HRV in hospitalized children with clinical manifestations of acute lower respiratory infection in Paraguay. Nasopharyngeal aspirates were collected from 101 children under 5 years of age, hospitalized with symptoms of acute lower respiratory infection, between May 2010 and December 2011, at the largest public pediatric hospital in the Central Department of Paraguay. Detection was performed by a real-time polymerase chain reaction, followed by conventional amplification of the VP4/VP2 genomic region, sequencing, and phylogenetic analysis. Rhinovirus was detected in 33.7% of the samples. Amplification of 18 samples showed the presence of all three species (HRV-A, -B, and -C). Different genotypes were found for each species: 11 for HRV-A (-9, -12, -22, -30, -36, -43, -59, -61, -68, -88, and -89), one for HRV-B (-4), and four for HRV-C (-C2, -C3, -C6, and -C9). In South America, information about HRV diversity is scarce. This is the first report on HRV genotype diversity in South America.

译文

人类犀牛病毒 (HRV) 是一种单链,正义RNA病毒,与儿童轻度上呼吸道感染有关。这项研究的目的是对巴拉圭有急性下呼吸道感染临床表现的住院儿童的HRV循环基因型进行分子表征和基于系统发育的分类。从巴拉圭中央最大的公立儿科医院的101名5岁以下儿童中收集了鼻咽抽吸物,这些儿童在2010年5月至2011年12月之间因急性下呼吸道感染而住院。通过实时聚合酶链反应进行检测,然后对VP4/VP2基因组区域进行常规扩增,测序和系统发育分析。在33.7% 个样品中检测到鼻病毒。18个样品的扩增显示存在所有三种物种 (hrv-a,-B和-C)。每个物种发现了不同的基因型: hrv-a为11 (-9,-12,-22,-30,-36,-43,-59,-61,-68,-88和-89),hrv-b (-4),四个用于hrv-c (-C2、-C3、-C6和-C9)。在南美,有关HRV多样性的信息很少。这是关于南美洲HRV基因型多样性的第一份报告。

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