Rabbits of allotype a1a3 were injected on days 0, 2, and 4 with mixtures containing equal amounts of pigeon erythrocytes (Prbc) coupled to para-azobenzenearsonate (AA) and to para-azobenzene-N-trimethylammonium (TMA). On day 6, the allotypes of antibody from plaque-forming cells (PFC) of the blood were determined by observing the inhibition of plaque formation by anti-allotype sera. Anti-AA PFC appeared to consist for the most part of cells making antibody of allotype a1 since 65% of them were inhibited by anti-a1 serum and only 8% by anti-a3. Anti-TMA PFC, on the other hand, appeared to consist mostly of cells making antibody of allotype a3, since less than 1% of them were inhibited by anti-a1 but 47% by anti-a3. Antibody allotype for spleen PFC was also determined on day 6 and was similar to that found for blood PFC. Anti-AA PFC were inhibited 74% by anti-a1 serum and 15% by anti-a3 whereas anti-TMA PFC were inhibited 19% by anti-a1 and 43% by anti-a3. Serum hemolysin specific for AA hapten from a1a3 animals was also strongly inhibited by anti-a1 serum but not by anti-a3 whereas the converse was true for hemolysin against TMA hapten. The a1a3 rabbits, in whcih the anti-AA was restricted to allotype a1, were mated to produced homozygous a3a3 animals. When the PFC and serum antibodies of these a3a3 offspring were examined by specific inhibition, the anti-AA activity was found to be of allotype a3 rather than being a-negative. The number of anti-AA PFC in the blood of a3a3 rabbits was lower than that in blood of a1a3 or a1a1 animals. In addition, the TMA hapten appeared to inhibit the response to the AA hapten. Thus a1a3 rabbits immunized with AA-Prbc alone had 14-fold more anti-AA PFC or 18-fold higher anti-AA hemolysin titer than a3a3 animals immunized with both AA-Prbc and TMA-Prbc. Our results are discussed in relation to various explanations which have been offered for an imbalance of allotypes in a given antibody.

译文

在第0、2和4天,向同种异体a1a3兔子注射含有等量的鸽子红细胞 (Prbc) 的混合物,该混合物与对偶氮苯硬脂酸酯 (AA) 和对偶氮苯-N-三甲基铵 (TMA) 偶联。在第6天,通过观察抗异体血清对斑块形成的抑制作用,确定了来自血液中斑块形成细胞 (PFC) 的抗体的同种异型。抗AA PFC似乎由大部分细胞组成,产生同种异型a1抗体,因为其中65% 被anti-a1血清抑制,仅被anti-a3 8%。另一方面,抗TMA PFC似乎主要由产生同种异体a3抗体的细胞组成,因为其中少于1% 的细胞被anti-a1抑制,但47% 被anti-a3抑制。脾脏PFC的抗体同种异型也在第6天测定,与血液PFC相似。抗AA PFC 74% 被anti-a1血清抑制,15% 被anti-a3抑制,而抗TMA PFC 19% 被anti-a1抑制,43% 被anti-a3抑制。来自a1a3动物的AA半抗原特异性血清溶血素也被anti-a1血清强烈抑制,但未被anti-a3抑制,而针对TMA半抗原的溶血素则相反。将抗AA仅限于同种异型a1的a1a3兔交配产生纯合的a3a3动物。当通过特异性抑制检查这些a3a3后代的PFC和血清抗体时,发现抗AA活性是同种异体a3,而不是a阴性。a3a3兔血液中抗AA PFC的数量低于a1a3或a1a1动物的血液。此外,TMA半抗原似乎抑制了对AA半抗原的反应。因此,仅用aa-prbc免疫的a1a3兔的抗AA PFC或抗AA溶血素滴度比用aa-prbc和tma-prbc免疫的a3a3动物高14倍。我们讨论了有关给定抗体中同种异体失衡的各种解释的结果。

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