The interactions between nerve fibres from para- and orthosympathetic ganglia and adreno-cortical and -medullary cells were studied in joint cultures using explanted guinea-pig ciliary and sympathetic chain ganglia and enzyme-dispersed rat adrenal gland cells. Nerve fibres from both para- and orthosympathetic ganglia made only transitory contact with cortical cells, but consistently formed associations with isolated chromaffin cells which lasted for up to 10 days. Contacts between axons and chromaffin cells often showed particularly large varicosities and frequently withstood severe tests of durability from pulls of the fibre or the cell or both. By correlating phase contrast and catecholamine histochemistry (Falck-Hillarp method) it was shown that sympathetic fibres forming long-lasting contacts with chromaffin cells were adrenergic. The functional implications of the ability of autonomic nerves to distinguish between adreno-cortical and -medullary cells and the lack of specificity shown by the para- and orthosympathetic neurons during formation of long-lasting associations with chromaffin cells are discussed.

译文

使用外植的豚鼠睫状和交感链神经节以及酶分散的大鼠肾上腺细胞,在联合培养物中研究了来自对侧和正交感神经节的神经纤维与肾上腺皮质和髓质细胞之间的相互作用。来自副神经节和正交感神经节的神经纤维仅与皮质细胞短暂接触,但始终与分离的嗜铬细胞形成关联,持续长达10天。轴突和嗜铬细胞之间的接触通常显示出特别大的静脉曲张,并且经常经受住纤维或细胞或两者的拉力对耐久性的严格测试。通过关联相衬和儿茶酚胺组织化学 (Falck-Hillarp方法),表明与嗜铬细胞形成持久接触的交感神经纤维是肾上腺素能的。讨论了自主神经区分肾上腺皮质细胞和髓质细胞的能力的功能含义,以及在与嗜铬细胞形成长期关联的过程中,副神经和直系神经神经元表现出的缺乏特异性。

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