The actinomycetes strain, lut0910, was isolated from polluted soil and identified as the Rhodococcus species with 99% similarity based on the sequence analysis of 16S recombinant DNA. The extract of this strain demonstrated in vivo and in vitro antitumor activity. The treatment of two human cancer cell lines, hepatocellular carcinoma HepG2 and cervical carcinoma Hela cells, with the lut0910 extract caused the delay in cell propagation in a dose-dependent manner with an IC50 of 73.39 and 33.09 µg/mL, respectively. Also, the oral administration of lut0910 extract to the mice with a solid tumor resulted in the inhibition of tumor growth in comparison with a placebo group. The thymus and spleen indexes were significantly increased in mice groups treated with the lut0910 extract. The histopathological changes of the tumor tissues showed that there were massive necrotic areas in the tumor tissues after treatment with different doses of the lut0910 extract. Our result would provide a new way and potent source for development of new anticancer agent from the polluted environment.

译文

从污染的土壤中分离出放线菌菌株lut0910,并根据对16s重组DNA的序列分析将其鉴定为具有99% 相似性的红球菌属。该菌株的提取物具有体内和体外抗肿瘤活性。用lut0910提取物处理两种人类癌细胞系,肝细胞癌HepG2和宫颈癌Hela细胞,以剂量依赖性方式引起细胞增殖的延迟,IC50分别为73.39和33.09 μ g/mL。此外,与安慰剂组相比,向患有实体瘤的小鼠口服lut0910提取物可抑制肿瘤生长。用lut0910提取物处理的小鼠组的胸腺和脾脏指数显着增加。肿瘤组织的组织病理学变化表明,用不同剂量的lut0910提取物治疗后,肿瘤组织中存在大量坏死区域。我们的结果将为从污染环境中开发新型抗癌剂提供新的途径和有效的来源。

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