The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear. In our previous study, a homologous gene of PacC/Rim101 was identified, and PacC-silenced strains of the agaricomycete Ganoderma lucidum were constructed. In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains. In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains. Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression. The ability of PacC to bind to the promoters of glucan synthase-encoding genes confirms that PacC transcriptionally regulates these genes.

译文

转录因子PacC/Rim101参与了许多真菌的环境pH适应,发育和次生代谢,但PacC/Rim101是否有助于真菌对环境胁迫的适应仍不清楚。在我们先前的研究中,鉴定了PacC/Rim101的同源基因,并构建了伞菌灵芝的PacC沉默菌株。在这项研究中,我们进一步研究了灵芝中PacC的功能,发现与野生型 (WT) 和空载体控制 (CK) 菌株相比,PacC沉默菌株对环境胁迫 (如渗透应激,氧化应激和细胞壁应激) 高度敏感。此外,细胞壁结构的透射电子显微镜图像显示,经PacC沉默的菌株的细胞壁比WT和CK菌株的细胞壁薄 (约25-30%)。细胞壁组成的进一步分析表明,PacC沉默菌株中的 β-1,3-葡聚糖含量仅为WT菌株的约78-80%,并且 β-1,3-葡聚糖含量的变化与葡聚糖合酶基因表达的下调一致。PacC与葡聚糖合酶编码基因启动子结合的能力证实了PacC在转录上调节这些基因。

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