The aim of this study was to detect the seasonal expressions of oxytocin (OT), oxytocin receptor (OTR), extracellular signal-regulated kinase1 and 2 (ERK1/2) and phospho-ERK1/2 (pERK1/2) in the epididymis of the wild ground squirrels (Citellus Dauricus Brandt) during the breeding season and non-breeding season. Histological results showed that size, weight, cell number and lumen diameter of epididymis underwent acute seasonal changes, which were all peaked in the breeding season. Immunohistochemical results suggested that strong staining of OT, OTR, ERK1/2, and pERK1/2 were observed in the epithelial layer in the whole epididymis, along with intense OT and OTR signal in smooth muscle cell (Smc) in caudal epididymis in the breeding season. The protein expression levels of OTR, ERK1/2, and pERK1/2 in the epididymis were higher in the breeding season than those of the non-breeding season. Besides, hormone assay revealed that there was no significant serum concentration of OT in these two periods, while epididymal concentration showed higher value in the breeding season. In summary, the identified localization and local concentration of OT in the epididymis in the wild ground squirrel suggested that epididymis may assume as a source of OT, and OT could act via OTR to activate ERK1/2 signaling to regulate seasonal epididymal functions.

译文

本研究的目的是检测催产素 (OT),催产素受体 (OTR) 的季节性表达,在繁殖季节和非繁殖季节,野生地松鼠 (Citellus Dauricus Brandt) 附睾中的细胞外信号调节激酶1和2 (ERK1/2) 和phospho-ERK1/2 (pERK1/2)。组织学结果表明,附睾的大小,重量,细胞数和管腔直径均发生了急性季节变化,在繁殖季节均达到峰值。免疫组织化学结果表明,在繁殖季节,在整个附睾的上皮层中观察到OT,OTR,ERK1/2和pERK1/2的强烈染色,以及尾附睾平滑肌细胞 (Smc) 中的强烈OT和OTR信号。在繁殖季节,附睾中OTR,ERK1/2和pERK1/2的蛋白表达水平高于非繁殖季节。此外,激素测定表明,在这两个时期,OT的血清浓度均不明显,而附睾浓度在繁殖季节显示出较高的值。总之,野生地松鼠附睾中OT的定位和局部浓度表明,附睾可能是OT的来源,OT可以通过OTR激活ERK1/2信号来调节季节性附睾功能。

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