A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.

译文

筛选豌豆荚cDNA文库中的木质化组织特异性序列。编码hsp70热休克蛋白C末端区域的cDNA克隆 (pLP19) 仅与发生pod木质化的豌豆系的pod mRNA杂交。热休克在豌豆和鹰嘴豆植物的叶,茎和根中诱导pLP19的表达。在与plp19相同的基因衍生的cdna中观察到四个不同的聚 (A) 加成位点。该基因已完全测序; 与大多数hsp70基因不同,它不包含内含子。5 '-侧翼序列包含热休克元件和其他潜在的调控序列。

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