Osteoporosis is an important skeletal disease characterized by bone weakness and high risk of fracture in postmenopausal women. Tea consumption is known to play an important role in the prevention or alleviation of osteoporosis. However, the therapeutic effects of aqueous extracts of white tea (WT) have not been evaluated in osteoporosis rat models. The aim of this study was to investigate the potential anti-osteoporotic role of WT in ovariectomized (OVX) rats. WT was given orally at 0.5% w/v doses for 12 weeks in OVX rats. Biochemical parameters in blood samples, bone tartrate-resistant acid phosphatase (TRAP), C-terminal telopeptide of type 1 collagen (CTX) and estradiol levels were evaluated. Bone mineral density and bone mineral content values were measured in the left femur. In addition to histopathological examination, osteolcalcin, osteopontin and TUNEL levels were determined. OVX group data demonstrated that bone loss occurred by thinning of the metaphyseal growth plates of the femur. Similarly, the levels of TRAP and CTX, markers of osteoclastic activity, were found to be high concurrently with a decrease in femoral bone mineral density. In addition, increased osteolcalcin and osteopontin levels were present in the metaphyseal growth zones. On the other hand, while TRAP and CTX levels were suppressed in the OVX-WT group, bone mineral content increased. In ad-dition, TUNEL, osteocalcin and osteopontin positivity decreased in the right femoral metaphysis growth zones, proliferating zone and resting zone cells. These results showed that chronic WT consumption has a protective effect by reducing bone resorption in OVX-induced osteoporotic rats.

译文

骨质疏松症是一种重要的骨骼疾病,其特征是绝经后妇女的骨骼无力和高骨折风险。众所周知,饮茶在预防或减轻骨质疏松症中起着重要作用。然而,尚未在骨质疏松症大鼠模型中评估白茶 (WT) 水提取物的治疗作用。这项研究的目的是研究WT在去卵巢 (OVX) 大鼠中的潜在抗骨质疏松作用。在OVX大鼠中以0.5% w/v剂量口服WT 12周。评估血液样本中的生化参数,抗骨酒石酸酸性磷酸酶 (TRAP),1型胶原蛋白的C末端肽 (CTX) 和雌二醇水平。测量左股骨的骨矿物质密度和骨矿物质含量值。除组织病理学检查外,还确定了骨钙素,骨桥蛋白和TUNEL水平。OVX组数据表明,股骨干phy端生长板变薄会导致骨质流失。同样,发现破骨细胞活性标志物TRAP和CTX的水平很高,同时股骨骨矿物质密度降低。此外,在干phy端生长区域中存在骨钙素和骨桥蛋白水平升高。另一方面,尽管OVX-WT组的TRAP和CTX水平受到抑制,但骨矿物质含量增加。另外,TUNEL,骨钙素和骨桥蛋白阳性在右股骨干端生长区,增殖区和静息区细胞中降低。这些结果表明,长期消耗WT通过减少OVX诱导的骨质疏松大鼠的骨吸收而具有保护作用。

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