Osteopontin (OPN) is a major glycosylated phosphoprotein in bone matrix and is produced by several cells including osteoblasts, osteoclasts and macrophages. OPN levels increase in active sites of bone metabolism. Recently, several bone-related proteins were identified in gingival crevicular fluid (GCF) to seek markers of alveolar bone resorption in periodontal disease. In this study, we investigated the existence of OPN in GCF and the correlation between OPN level in GCF and probing depth (PD) of sampling sites in 98 periodontitis patients and 35 healthy subjects. An immunoblotting analysis using 10% polyacrylamide gel showed that two forms of OPN with molecular masses of 54 and 66 kDa and several degraded fragments were detected in most GCF samples from diseased sites (PD > 4 mm). In GCF samples from healthy sites (PD < or = 3 mm), only one form (54 kDa) was observed, but any degraded fragments were not detected. When OPN amounts in GCF samples were determined by ELISA, a weak. but significant correlation was observed between OPN amount in GCF and PD (r=0.32, p=0.0013). These results demonstrate that OPN exists in GCF and that OPN level in GCF increases with the progression of periodontal disease.

译文

骨桥蛋白 (OPN) 是骨基质中主要的糖基化磷蛋白,由成骨细胞,破骨细胞和巨噬细胞等多种细胞产生。骨代谢活性部位的OPN水平增加。最近,在龈沟液 (GCF) 中发现了几种与骨相关的蛋白质,以寻找牙周病中牙槽骨吸收的标志物。在这项研究中,我们调查了98例牙周炎患者和35例健康受试者的GCF中OPN的存在以及GCF中OPN水平与采样点探测深度 (PD) 之间的相关性。使用10% 聚丙烯酰胺凝胶的免疫印迹分析表明,在来自患病部位的大多数GCF样品中检测到两种形式的具有54和66 kda的分子量的OPN和几个降解的片段 (PD> 4毫米)。在来自健康位点 (PD <或 = 3毫米) 的GCF样品中,仅观察到一种形式 (54 kda),但未检测到任何降解的片段。当通过ELISA测定GCF样品中的OPN量时,弱。但是在GCF中的OPN量与PD之间观察到显着的相关性 (r = 0.32,p = 0.0013)。这些结果表明,GCF中存在OPN,GCF中的OPN水平随着牙周病的进展而增加。

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