Osteopontin that associates with metabolism of calcium is one of the important factors in the development and prognosis of human breast cancer. The aim of this study was to detect potential binding partners of osteopontin to illustrate its functional mechanism. By screening a human breast cDNA library with a bacterial two-hybrid system, apolipoprotein D was isolated as a novel interacting protein of osteopontin. This interaction was confirmed by mammalian two-hybrid assay and co-immunoprecipitation. To elucidate the influence of ApoD on cellular neoplastic specifications, adhesion, soft agar, invasion and MTT growth assays were performed with Rama37 cells. The results revealed that expression of apolipoprotein D in Rama37 cells could significantly inhibit the malignant phenotype in osteopontin-transformed Rama37 cells. These findings provide better knowledge of the osteopontin signaling pathway and suggest that apolipoprotein D could be a prospective therapeutic agent for human breast and/or other carcinomas.

译文

:与钙代谢相关的骨桥蛋白是人类乳腺癌发展和预后的重要因素之一。这项研究的目的是检测骨桥蛋白的潜在结合伴侣,以说明其功能机制。通过用细菌双杂交系统筛选人乳腺cDNA文库,分离出载脂蛋白D作为骨桥蛋白的新型相互作用蛋白。这种相互作用已通过哺乳动物双杂交测定和免疫共沉淀得到了证实。为了阐明ApoD对细胞赘生物规格的影响,对Rama37细胞进行了粘附,软琼脂,侵袭和MTT生长测定。结果表明载脂蛋白D在Rama37细胞中的表达可以显着抑制骨桥蛋白转化的Rama37细胞的恶性表型。这些发现提供了对骨桥蛋白信号传导途径的更好的了解,并表明载脂蛋白D可能是人乳腺癌和/或其他癌症的前瞻性治疗剂。

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