We assessed the pathological changes of articular cartilage and subchondral bone on different locations of the knee after extracorporeal shockwave therapy (ESWT) in early osteoarthritis (OA). Rat knees under OA model by anterior cruciate ligament transaction (ACLT) and medial meniscectomy (MM) to induce OA changes. Among ESWT groups, ESWT were applied to medial (M) femur (F) and tibia (T) condyles was better than medial tibia condyle, medial femur condyle as well as medial and lateral (L) tibia condyles in gross osteoarthritic areas (p<0.05), osteophyte formation and subchondral sclerotic bone (p<0.05). Using sectional cartilage area, modified Mankin scoring system as well as thickness of calcified and un-calcified cartilage analysis, the results showed that articular cartilage damage was ameliorated and T+F(M) group had the most protection as compared with other locations (p<0.05). Detectable cartilage surface damage and proteoglycan loss were measured and T+F(M) group showed the smallest lesion score among other groups (p<0.05). Micro-CT revealed significantly improved in subchondral bone repair in all ESWT groups compared to OA group (p<0.05). There were no significantly differences in bone remodeling after ESWT groups except F(M) group. In the immunohistochemical analysis, T+F(M) group significant reduced TUNEL activity, promoted cartilage proliferation by observation of PCNA marker and reduced vascular invasion through observation of CD31 marker for angiogenesis compared to OA group (P<0.001). Overall the data suggested that the order of the effective site of ESWT was T+F(M) ≧ T(M) > T(M+L) > F(M) in OA rat knees.

译文

:我们评估了在早期骨关节炎(OA)中进行体外冲击波治疗(ESWT)后,膝关节不同部位的关节软骨和软骨下骨的病理变化。 OA模型下的大鼠膝盖通过前交叉韧带交易(ACLT)和内侧半月板切除术(MM)诱导OA改变。在ESWT组中,将ESWT应用于大骨关节炎地区的内侧(M)股骨(F)和胫骨(T)dy优于内侧胫骨con,内侧股骨con以及胫骨内侧和外侧(L)((p < 0.05),骨赘形成和软骨下硬化骨(p <0.05)。使用软骨截面面积,改良的Mankin评分系统以及钙化和非钙化软骨的厚度分析,结果表明,与其他部位相比,TF(M)组对关节软骨的损害得到了最大程度的保护(p <0.05 )。测量可检测到的软骨表面损伤和蛋白聚糖损失,TF(M)组在其他组中的病变评分最小(p <0.05)。 Micro-CT显示,与OA组相比,所有ESWT组的软骨下骨修复均显着改善(p <0.05)。除F(M)组外,ESWT组的骨重塑没有显着差异。在免疫组织化学分析中,与OA组相比,TF(M)组通过观察PCNA标记显着降低了TUNEL活性,促进了软骨增殖,并且通过观察CD31标记的血管生成降低了血管侵袭(P <0.001)。总体而言,数据表明,OA大鼠膝关节ESWT有效部位的顺序为T F(M)≥T(M)> T(ML)> F(M)。

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