Chlorogenic acid (CGA), which is a natural compound found in various plants, has been reported to exert notable anti‑inflammatory activities. The present study investigated the effects and underlying mechanism of CGA on interleukin (IL)‑1β‑induced osteoarthritis (OA) chondrocytes. An in vitro OA‑like chondrocyte model was established using IL‑1β‑stimulated human SW‑1353 chondrocytes. Cell viability was assessed using an MTT assay. Nitric oxide (NO) and IL‑6 production were evaluated by Griess reaction and ELISA, respectively. The expression levels of inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), cyclooxygenase 2 (COX‑2), collagen II, matrix metalloproteinase (MMP)‑13, p65 nuclear factor (NF)‑κB and inhibitor‑κBα were detected by western blot analysis. The results indicated that CGA reversed IL‑1β‑induced increases in iNOS/NO, IL‑6, MMP‑13 and COX‑2/PGE2 production, and reversed the IL‑1β‑mediated downregulation of collagen II. In addition, the data suggested that CGA was capable of inhibiting the IL‑1β‑induced inflammatory response, at least partially via the NF‑κB signaling pathway. In conclusion, CGA may be considered a suitable candidate agent in the treatment of OA.

译文

绿原酸(CGA)是多种植物中发现的天然化合物,据报道具有显着的抗炎活性。本研究调查了CGA对白介素(IL)-1β诱导的骨关节炎(OA)软骨细胞的影响及其潜在机制。使用IL-1β刺激的人类SW-1353软骨细胞建立了类似OA的体外软骨细胞模型。使用MTT测定法评估细胞生存力。一氧化氮(NO)和IL-6的产生分别通过Griess反应和ELISA进行评估。诱导型一氧化氮合酶(iNOS),前列腺素E2(PGE2),环氧合酶2(COX‑2),胶原蛋白II,基质金属蛋白酶(MMP)-13,p65核因子(NF)‑κB和抑制剂‑κBα的表达水平分别为通过蛋白质印迹分析检测。结果表明,CGA逆转了IL-1β诱导的iNOS / NO,IL-6,MMP-13和COX-2-PGE2产生的增加,并逆转了IL-1β介导的II型胶原的下调。此外,数据表明CGA能够至少部分地通过NF-κB信号传导途径抑制IL-1β诱导的炎症反应。总之,CGA可被认为是治疗OA的合适候选药物。

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