The antioxidant activities of water extract, methanol extract, ethyl acetate, and n-BuOH fractions of methanol extract from Lysimachia foenum-graecum Hance were investigated in this study. Various methods, such as the total antioxidant capacity measured by phosphomolybdenum method, scavenging activities towards 1,1-diphenyl-2-picryhydrazyl (DPPH) radical, superoxide anion radical, and hydroxyl radical, were established in in vitro systems. The amounts of total phenolics and total flavonoids in the extracts and fractions were also determined by spectrophotometric methods. The results showed that all the extracts and fractions exhibited antioxidant and radical-scavenging activities at different magnitudes of potency. The decreasing order of antioxidant and radical-scavenging activities among the extracts assayed through all the four methods were found to be ethyl acetate fraction>n-BuOH fraction>methanol extract>water extract. This similar order of the amounts of total phenolics and total flavonoids shows that the extent of antioxidant and radical-scavenging activities is in accordance with the amounts of phenolics and flavonoids present in extracts and fractions. The extracts of L. foenum-graecum Hance might be valuable antioxidant natural sources and seemed to be applicable in both healthy medicine and food industry.

译文

:本研究研究了金钱草-香茅的水提取物,甲醇提取物,乙酸乙酯和甲醇提取物的n-BuOH级分的抗氧化活性。在体外系统中建立了多种方法,例如通过磷钼法测量的总抗氧化能力,对1,1-二苯基-2-吡啶并肼基(DPPH)自由基,超氧阴离子自由基和羟基自由基的清除活性。提取物和馏分中总酚和总黄酮的含量也通过分光光度法测定。结果表明,所有提取物和馏分在不同的效价下均表现出抗氧化和清除自由基的活性。通过四种方法测定的提取物中抗氧化剂和自由基清除活性的降低顺序为:乙酸乙酯级分> n-BuOH级分>甲醇提取液>水提取液。总酚和总类黄酮含量的这一相似顺序表明,抗氧化剂和自由基清除活性的程度与提取物和馏分中酚和类黄酮的含量一致。香菇(L. foenum-graecum Hance)的提取物可能是有价值的抗氧化剂天然来源,似乎适用于健康医学和食品工业。

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