The objective of this study was to evaluate the effects of ultrasound-mediated analyte diffusion on permeability of normal, benign, and cancerous human lung tissue in vitro and to find more effective sonophoretic (SP) delivery in combination with the optical clearing agents (OCAs) method to distinguish normal and diseased lung tissues. The permeability coefficients of SP in combination with OCAs diffusion in lung tissue were measured with Fourier-domain optical coherence tomography (FD-OCT). 30% glucose and SP with a frequency of 1 MHz and an intensity of 0.80  W/cm2 over a 3 cm probe was simultaneously applied for 15 min. Experimental results show that the mean permeability coefficients of 30% glucose/SP were found to be (2.01±0.21)×10(-5)  cm/s from normal lung (NL) tissue, (2.75±0.28)×10(-5)  cm/s from lung benign granulomatosis (LBG) tissue, (4.53±0.49)×10(-5)  cm/s from lung adenocarcinoma tumor (LAT) tissue, and (5.81±0.62)×10(-5)  cm/s from lung squamous cell carcinoma (LSCC) tissue, respectively. The permeability coefficients of 30% glucose/SP increase approximately 36.8%, 125.4%, and 189.1% for the LBG, LAT, and LSCC tissue compared with that for the NL tissue, respectively. There were statistically significant differences in permeability coefficients of 30% glucose/SP between LBG and NL tissue (p<0.05), between LAT and NL tissue (p<0.05), and between LSCC and NL tissue (p<0.05). The results suggest that the OCT functional imaging technique to combine an ultrasound-OCAs combination method could become a powerful tool in early diagnosis and monitoring of changed microstructure of pathologic human lung tissue.

译文

:这项研究的目的是评估超声介导的分析物扩散对正常,良性和癌性人肺组织的体外渗透性的影响,并与光学清除剂(OCA)结合发现更有效的超声导入(SP)方式)区分正常和患病肺组织的方法。用傅里叶域光学相干断层扫描(FD-OCT)测量SP的渗透系数以及OCA在肺组织中的扩散。同时在3 cm探针上施加30%的葡萄糖和SP,频率为1 MHz,强度为0.80 W / cm2,持续15分钟。实验结果表明,与正常肺组织相比,30%葡萄糖/ SP的平均渗透系数为(2.01±0.21)×10(-5)cm / s,(2.75±0.28)×10(-5)肺良性肉芽肿病(LBG)组织的cm / s,肺腺癌肿瘤(LAT)组织的(4.53±0.49)×10(-5)cm / s和(5.81±0.62)×10(-5)cm /分别来自肺鳞状细胞癌(LSCC)组织。与NL组织相比,LBG,LAT和LSCC组织的30%葡萄糖/ SP的渗透系数分别增加了约36.8%,125.4%和189.1%。 LBG和NL组织之间(p <0.05),LAT和NL组织之间(p <0.05)以及LSCC和NL组织之间(p <0.05)的30%葡萄糖/ SP的通透性系数在统计学上有显着差异。结果表明,OCT功能成像技术结合超声-OCAs组合方法可能成为早期诊断和监测病理性人肺组织微观结构变化的有力工具。

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