Mice lacking T cell receptor alpha chain (TCRalpha(-/-)) develop inflammation of the colon. We have examined the effect of this inflammation on the colonic epithelium by studying markers of epithelial cuff, enteroendocrine, and immune cell differentiation. Using immunohistochemical techniques, colons were compared in normal C57/BL6 and murine TCR alpha(-/-) mice aged 2 and 3 weeks and 3-11 months. TCR alpha(-/-) mice aged 3-11 months had histologic evidence of inflammation with increased expression of CD45, CD4+, CD8+, and B220+ cells and a decrease in expression of IgA+ cells. There was a decrease in the number of cholecystokinin, serotonin, and neurotensin enteroendocrine expressing cells in the colon of TCR alpha(-/-) mice. These changes were not present in 2-3-week-old suckling/weaning mice. In contrast, peptide tyrosine tyrosine (PYY), glucagon-like peptide-1, and gastrin expression did not change and small intestinal enteroendocrine cells remained unaltered. The change in colonic enteroendocrine cell expression appears to be a specific response, since only a subset of these cells was altered, and the epithelium was intact by histologic analysis. The absence of functional T cells in TCR alpha(-/-) colon has a marked effect on differentiation of a specific subpopulation of enteroendocrine cells, prior to loss of integrity of the epithelium.

译文

:缺乏T细胞受体α链(TCRalpha(-/-))的小鼠发展成结肠的炎症。我们已经通过研究上皮袖带,肠内分泌和免疫细胞分化的标志物检查了这种炎症对结肠上皮的影响。使用免疫组织化学技术,比较了2周,3周和3-11个月大的正常C57 / BL6和鼠TCR alpha(-/-)小鼠的结肠。 3-11个月大的TCR alpha(-/-)小鼠具有炎症的组织学证据,CD45,CD4,CD8和B220细胞表达增加,而IgA细胞表达减少。在TCR alpha(-/-)小鼠结肠中,胆囊收缩素,血清素和神经降压素肠内分泌表达细胞的数量有所减少。这些变化在2至3周大的哺乳/断奶小鼠中不存在。相反,肽酪氨酸酪氨酸(PYY),胰高血糖素样肽-1和胃泌素的表达没有改变,小肠肠内分泌细胞保持不变。结肠肠内分泌细胞表达的变化似乎是一种特异性反应,因为这些细胞中只有一部分被改变,并且通过组织学分析完整上皮。在失去上皮的完整性之前,TCR alpha(-/-)结肠中功能性T细胞的缺失对肠内分泌细胞特定亚群的分化具有明显的影响。

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