The aim of our study was to determine, using histological and stereological methods, whether photoperiodism has any impact on the effects that chronic (three-month long) exposure to LF-EMF (50Hz) has on morphological characteristics on rat's pineal gland. The experiment was performed on 48 Mill Hill male rats (24 experimental and 24 control). Upon birth, 24 rats were exposed for 7h a day, 5 days a week for 3 months to LF-EMF (50 Hz, 50-500microT, 10V/m). In the winter (short days, long nights), the activity of the pineal gland and neuroendocrine sensitivity is increased. The study was performed both during summer and winter, following the identical protocol. After sacrifice of animals, samples of pineal gland were processed for HE staining and then were analyzed using the methods of stereology. The most significant changes in epiphysis in the first group of animals in wintertime are: altered glandular feature, hyperemia, reduced pinealocytes with pale pink, poor cytoplasm and irregular, stick-form nuclei. In the second group (II) pinealocytes are enlarged, with vacuolated cytoplasm and hyper chromatic, enlarged nucleus. Morphological changes of pineal gland at rats in the summertime were not as intense as in the winter and finding of the gland in the group II is compatible with those from the control group. Stereological results show both in winter and summer in the first group the decrease of volume density of pinealocytes, their cytoplasm and nuclei and in the second group in winter increase the volume density of pinealocytes, cytoplasm and nuclei, while in the second group the results in summertime are equal to those from the control group. Photoperiodism is modifier of effect of LF-EMF on morphological structure of pineal gland, because the gland recovery is incomplete in winter and reversible in summer.

译文

:我们的研究目的是使用组织学和立体学方法确定光周期是否对长期(三个月以上)长期暴露于LF-EMF(50Hz)的大鼠松果体形态特征有影响。实验在48只Mill Hill雄性大鼠上进行(24只实验动物和24只对照组)。出生后,将24只大鼠每天,每周5天,每天7h,3个月暴露于LF-EMF(50 Hz,50-500microT,10V / m)。在冬季(短日,长夜),松果体的活动和神经内分泌敏感性增加。按照相同的方案,在夏季和冬季均进行了该研究。处死动物后,对松果体样品进行HE染色处理,然后使用立体学方法进行分析。冬季,第一组动物的骨physi最明显的变化是:腺体特征改变,充血,松果体细胞减少,呈浅粉红色,胞质差,核形不规则,呈棒状。在第二组(II)中,松果体细胞增大,具有空泡的细胞质和高色度,扩大的核。夏季,大鼠松果体的形态学变化不像冬季那样强烈,并且在第二组中发现的腺体与对照组的相符。立体学结果表明,第一组在冬季和夏季均降低了松果体细胞,其胞质和细胞核的体积密度,第二组在冬季中均升高了松果体细胞,细胞质和细胞核的体积密度,而第二组的结果则是夏季的时间与对照组的时间相同。光周期是LF-EMF对松果体形态结构影响的调节剂,因为冬季腺体恢复不完全,夏天可逆。

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