We previously reported that the BARF1 (BamH1-A right frame 1) gene product from Epstein-Barr Virus (EBV) may have oncogenic properties since injection into new-born rats of transfected cell lines resulted in the development of BARF1 expressing tumors, which were aggressive in the case of murine fibroblasts and transient in that of human B lymphocytes. As EBV has been associated with nasopharyngeal carcinoma (NPC) and evidence of BARF1 transcription in this cancer was emerging from our biopsy analyses, we examined the effects of BARF1 transfection into primate primary epithelial cells. The expression of the BARF1 open reading frame in primary monkey kidney epithelial cells led us to the establishment of continuously dividing lines. The BARF1 transfectants showed the major characteristics of immortalized cellsmorphological change, short cell doubling time, ability to divide at low cell density and continuous growth over 50 passages. Injection of BARF1 transfectants into nude mice did not induce any tumor. Established subclones were shown to be epithelial cells expressing known keratins as well as the BARF1 coded mRNA and protein. This is the first report indicating that expression of the BARF1 gene product in primary epithelial cells may contribute to the establishment of cell lines.

译文

我们先前曾报道,爱泼斯坦-巴尔病毒(EBV)的BARF1(BamH1-A右框架1)基因产物可能具有致癌特性,因为向新生大鼠转染的细胞系中注射导致表达BARF1的肿瘤的发展,鼠成纤维细胞具有侵袭性,人B淋巴细胞具有瞬时侵袭性。由于EBV与鼻咽癌(NPC)相关,并且从我们的活检分析中发现了该癌症中BARF1转录的证据,因此我们检查了BARF1转染到灵长类动物原代上皮细胞中的作用。 BARF1开放阅读框在原代猴肾上皮细胞中的表达使我们建立了连续分裂系。 BARF1转染子显示出永生化细胞形态变化,短细胞倍增时间,在低细胞密度下分裂的能力以及在50代以上连续生长的主要特征。向裸鼠中注射BARF1转染子不会诱导任何肿瘤。已建立的亚克隆显示为表达已知角蛋白以及BARF1编码的mRNA和蛋白质的上皮细胞。这是第一个报告,表明BARF1基因产物在原代上皮细胞中的表达可能有助于细胞系的建立。

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