BACKGROUND:Germ cells are the only cell type that can penetrate from one generation to next generation. At the early embryonic developmental stages, germ cells originally stem from primordial germ cells, and finally differentiate into functional gametes, sperm in male or oocyte in female, after sexual maturity. This study was conducted to investigate a large-scale expressed sequence tag (EST) analysis in chicken PGCs and compare the expression of the PGC ESTs with that of embryonic gonad. RESULTS:We constructed 10,851 ESTs from a chicken cDNA library of a collection of highly separated embryonic PGCs. After chimeric and problematic sequences were filtered out using the chicken genomic sequences, there were 5,093 resulting unique sequences consisting of 156 contigs and 4,937 singlets. Pearson chi-square tests of gene ontology terms in the 2nd level between PGC and embryonic gonad set showed no significance. However, digital gene expression profiling using the Audic's test showed that there were 2 genes expressed significantly with higher number of transcripts in PGCs compared with the embryonic gonads set. On the other hand, 17 genes in embryonic gonads were up-regulated higher than those in the PGC set. CONCLUSION:Our results in this study contribute to knowledge of mining novel transcripts and genes involved in germline cell proliferation and differentiation at the early embryonic stages.

译文

背景:生殖细胞是唯一可以从一代渗透到下一代的细胞类型。在胚胎发育的早期阶段,生殖细胞最初起源于原始生殖细胞,并在性成熟后分化为功能性配子,雄性精子或雌性卵母细胞。进行这项研究以调查鸡PGC中的大规模表达序列标签(EST)分析,并将PGC EST与胚胎性腺的表达进行比较。
结果:我们从鸡的高度分离的胚胎PGC的cDNA文库中构建了10,851个EST。使用鸡基因组序列过滤掉嵌合和有问题的序列后,共有5,093个得到的独特序列,由156个重叠群和4,937个单重态组成。 PGC和胚胎性腺集之间第二级的基因本体项的皮尔逊卡方检验没有意义。但是,使用Audic's测试进行的数字基因表达谱分析显示,与胚胎性腺相比,PGC中有2个基因表达显着,且转录本数量更高。另一方面,胚胎性腺中的17个基因上调的程度高于PGC中的基因。
结论:我们在这项研究中的结果有助于了解在胚胎早期阶段涉及生殖细胞增殖和分化的新转录本和基因。

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