The purpose of this study was to clarify the effects of nutrients on the gonadal development of male rats kept under constant darkness as a model of disturbed daily rhythm. The present study examined protein and vitamins, and their interactions. This study was based on three-way ANOVA; the three factors were lighting conditions, dietary protein and dietary vitamins, respectively. The levels of dietary protein were low or normal: 9% casein or 20% casein. The levels of dietary vitamins were low, normal or high: 1/3.3 of normal (AIN-93G diet) content, normal content, or three times the normal content, respectively. Other compositions were the same as those of the AIN-93G diet, and six kinds of experimental diet were prepared. Four-week-old rats (Fischer 344 strain) were kept under constant darkness or normal lighting (12-h light/dark cycle) for 4 wk. After 4 wk, the gonadal weights and serum testosterone content were evaluated. In the constant darkness groups (D-groups), the low-protein diet induced reduction of gonadal organ weights and serum testosterone concentrations. This reduction of gonadal organ weights was exacerbated by progressively higher levels of dietary vitamins. In the case of a normal-protein diet, the depression of gonadal development was not accelerated by high-vitamin intake. In the normal lighting groups (N-groups), the low-protein and high-vitamin diet slightly depressed gonadal development. These results suggest that the metabolism of protein and vitamins is different in rats being kept under constant darkness, and that excess dietary vitamins have an adverse effect on gonadal development in rats fed a low-protein diet.

译文

:本研究的目的是阐明养分对处于持续黑暗中的雄性大鼠性腺发育的影响,这是日常节律紊乱的模型。本研究检查了蛋白质和维生素及其相互作用。本研究基于三向方差分析;这三个因素分别是光照条件,饮食蛋白和饮食维生素。膳食蛋白水平低或正常:酪蛋白9%或酪蛋白20%。饮食中维生素的水平低,中或高:分别为正常(AIN-93G饮食)含量的1 / 3.3,正常含量或正常含量的三倍。其他组成与AIN-93G饮食相同,准备了6种实验饮食。将四周大的大鼠(Fischer 344株)保持在恒定的黑暗或正常光照下(12小时光照/黑暗周期)4周。 4周后,评估了性腺的重量和血清睾丸激素的含量。在恒定的黑暗组(D组)中,低蛋白饮食导致性腺器官重量和血清睾丸激素浓度降低。逐渐增加饮食中的维生素水平会加剧性腺器官重量的减少。在正常蛋白质饮食的情况下,高维生素摄入不会促进性腺发育的抑制。在正常照明组(N组)中,低蛋白和高维生素饮食会稍微抑制性腺发育。这些结果表明,在恒定黑暗条件下的大鼠中蛋白质和维生素的代谢不同,并且饮食中过量的维生素对低蛋白质饮食的大鼠的性腺发育有不利影响。

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