Reprogramming of a somatic nucleus to an induced pluripotent state can be achieved in vitro through ectopic expression of Oct4 (Pou5f1), Sox2, Klf4 and c-Myc. While the ability of these factors to regulate transcription in a pluripotent context has been studied extensively, their ability to interact with and remodel a somatic genome remains underexplored. Several recent studies have begun to provide mechanistic insights that will eventually lead to a more rational design and improved understanding of nuclear reprogramming.

译文

:通过异位表达Oct4(Pou5f1),Sox2,Klf4和c-Myc可以在体外实现将体细胞核重编程为诱导性多能状态。尽管已经广泛研究了这些因素在多能环境中调节转录的能力,但它们与体细胞基因组相互作用和重塑的能力仍未得到充分研究。最近的一些研究已经开始提供机械的见解,这些见解最终将导致更合理的设计和对核重编程的更好的理解。

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