The interaction between T4 DNA and basic polypeptides was observed using fluorescence microscopy. Free DNA molecules exhibited random Brownian motion accompanying the conformational change. With the addition of polycation, such as histone and polyarginine, DNA molecules tended to shrink to become spherical shapes. The persistent lengths and the distributions of long axis lengths of DNA-polyarginine complexes were determined from the video images at various polyarginine concentrations. It is demonstrated that the conformation of DNA changes in a biphasic manner in the presence of polyarginine.

译文

:使用荧光显微镜观察到T4 DNA和碱性多肽之间的相互作用。游离DNA分子随构象变化表现出随机的布朗运动。随着组蛋白和聚精氨酸等聚阳离子的加入,DNA分子趋于收缩成球形。 DNA-聚精氨酸复合物的持久长度和长轴长度的分布是从视频中不同聚精氨酸浓度确定的。证明在聚精氨酸存在下DNA的构象以双相方式改变。

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