The present study was undertaken to compare the effects of allyl mercaptan (AM), a major metabolite of garlic, with several garlic constituents and extracts on cytotoxicity, cholesterol synthesis and its secretion in Hep-G2 cells. The cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), and treated with 5, 25, 50, 125, 250 and 500 microg/ml of AM, diallyl disulfide (DD), diallyl trisulfide (DT), steam-distilled garlic oil (SD) or vinyl-dithiin oil of garlic (VD) for 4 h. At concentrations up to 50 microg/ml, no significant cytotoxic effect was found in any group, but at concentrations above 250 microg/ml, the cell viability decreased drastically in all groups compared to the control. The treatment of cells with 25 microg/ml (non-cytotoxic concentration) of AM, DD, DT, SD for 4 h significantly inhibited [3H]acetate incorporation into cholesterol compared to that of the control (P < 0.05). The secretion of cholesterol into the medium was also significantly decreased in all groups except for VD. The treatment of cells with those allium constituents had no effect on either [3H]acetate incorporation into fatty acids or [3H]glycerol incorporation into triglyceride or phospholipid.

译文

本研究旨在比较大蒜的主要代谢产物烯丙基硫醇 (AM) 与几种大蒜成分和提取物对细胞毒性,胆固醇合成及其在Hep-G2细胞中的分泌的影响。细胞在补充有10% 胎牛血清 (FBS) 的Dulbecco改良Eagle培养基 (DMEM) 中生长,并用5、25、50、125、250和500微克/毫升的AM,二烯丙基二硫化物 (DD),二烯丙基三硫化物 (DT) 处理,蒸汽蒸馏大蒜油 (SD) 或大蒜的乙烯基二硫肽油 (VD) 4小时。在高达50微克/毫升的浓度下,在任何组中没有发现显著的细胞毒性作用,但是在高于250微克/毫升的浓度下,与对照组相比,所有组中的细胞活力急剧下降。用25微克/毫升 (非细胞毒性浓度) 的AM,DD,DT,与对照组相比,SD 4 h显着抑制 [3H] 乙酸盐掺入胆固醇 (P <0.05)。除VD外,所有组的胆固醇分泌也显着降低。用那些葱属成分处理细胞对 [3H] 乙酸掺入脂肪酸或 [3H] 甘油掺入甘油三酸酯或磷脂。

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