The chromosome of Streptomyces coelicolor A3(2), a model organism for the genus Streptomyces, contains a cryptic type I polyketide synthase (PKS) gene cluster which was revealed when the genome was sequenced. The ca. 54-kb cluster contains three large genes, cpkA, cpkB and cpkC, encoding the PKS subunits. In silico analysis showed that the synthase consists of a loading module, five extension modules and a unique reductase as a terminal domain instead of a typical thioesterase. All acyltransferase domains are specific for a malonyl extender, and have a B-type ketoreductase. Tailoring and regulatory genes were also identified within the gene cluster. Surprisingly, some genes show high similarity to primary metabolite genes not commonly identified in any antibiotic biosynthesis cluster. Using western blot analysis with a PKS subunit (CpkC) antibody, CpkC was shown to be expressed in S. coelicolor at transition phase. Disruption of cpkC gave no obvious phenotype.

译文

:Streptomyces coelicolor A3(2)(Streptomyces属的一种模式生物)的染色体包含一个隐秘的I型聚酮化合物合酶(PKS)基因簇,该基因簇在对基因组进行测序时就显示出来。的ca。 54kb的簇包含三个大基因,编码PKS亚基的cpkA,cpkB和cpkC。在计算机分析中,该合成酶由一个加载模块,五个扩展模块和一个独特的还原酶(而不是典型的硫酯酶)作为末端结构域组成。所有酰基转移酶结构域都对丙二酰增量剂具有特异性,并具有B型酮还原酶。还可以在基因簇中鉴定出剪裁和调节基因。出乎意料的是,一些基因显示出与任何抗生素生物合成簇中未普遍鉴定的初级代谢产物基因高度相似。使用具有PKS亚基(CpkC)抗体的Western印迹分析,显示CpkC在过渡阶段在天蓝色链霉菌中表达。 cpkC的破坏没有明显的表型。

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