Synthetic peptides, peptides A (Arg-Leu-Tyr-Leu-Arg-Ile-Gly-Arg-Arg-NH(2)) and B (Arg-Leu-Arg-Leu-Arg-Ile-Gly-Arg-Arg-NH(2)), derived from the beetle Allomyrina dichotoma defensin, have antimicrobial activities. Immunotoxicological effect of these peptides was evaluated by cytotoxicity of mouse peritoneal macrophages. In addition, antigenicity of these peptides was studied by evaluating antibody responses in mice immunized with these peptides. The toxicity of peptide A toward mouse peritoneal cells was less than that of polymyxin B, when morphologically evaluated in a cytotoxicity test. Almost all of mice injected intraperitoneally (i.p.) with either peptide A or B at 50-150 mg/kg survived, whereas all mice injected i.p. with polymyxin B at the doses of more than 25 mg/kg died within 24 h. Interestingly, almost all of mice injected intravenously with these peptides at the doses of 10 and 25 mg/kg also survived. Furthermore, mice immunized with these peptides conjugated with keyhole limpet hemocyanin (KLH) showed little or negligible anti-peptide A or B antibody production, although anti-KLH antibody was significantly produced. The results indicated that peptides A and B were less cytotoxic than polymyxin B and also had poor antigenicity to produce specific antibody in mice.

译文

合成肽,肽A (Arg-Leu-Tyr-Leu-Arg-Ile-Gly-Arg-NH(2)) 和B (Arg-Leu-Arg-leg-Arg-Ile-Gly-Arg-NH(2)),衍生自甲虫二分蛋白防御素,具有抗菌活性。通过小鼠腹膜巨噬细胞的细胞毒性评估了这些肽的免疫毒理学作用。此外,通过评估用这些肽免疫的小鼠中的抗体反应,研究了这些肽的抗原性。在细胞毒性测试中进行形态学评估时,肽A对小鼠腹膜细胞的毒性小于多粘菌素B。几乎所有以50-150 mg/kg腹膜内 (i.p.) 注射肽A或B的小鼠均存活,而所有注射i.p.的小鼠均存活。多粘菌素b的剂量超过25 mg/kg,在24小时内死亡。有趣的是,几乎所有以10和25 mg/kg剂量静脉注射这些肽的小鼠也都存活了。此外,用这些与锁孔血蓝蛋白 (KLH) 缀合的肽免疫的小鼠显示出很少或可忽略的抗肽A或B抗体产生,尽管显着产生了抗KLH抗体。结果表明,肽A和肽B的细胞毒性低于多粘菌素B,并且在小鼠中产生特异性抗体的抗原性也较差。

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