Here we describe a single-step affinity column for purification of vectors based on adeno-associated virus type 5 (AAV5). A sialic-acid-rich protein called mucin was covalently attached to Sepharose and was found to bind AAV5 vectors. Elution with high salt efficiently recovered highly active vectors of greater purity than what is achieved with CsCl(2) sedimentation.

译文

:在此,我们描述了一步纯化亲和柱,用于基于5型腺伴随病毒(AAV5)纯化载体。富含粘液的唾液酸蛋白(称为粘蛋白)与Sepharose共价连接,并发现可与AAV5载体结合。高盐洗脱有效地回收了比CsCl(2)沉淀获得的纯度更高的高活性载体。

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