We have previously reported that a targeted anti-caries DNA vaccine, pGJA-P, induced accelerated and increased antibody responses compared with a non-targeted anti-caries DNA vaccine. Recently, pGJA-P/VAX, a new targeted anti-caries DNA vaccine for human trials, was constructed by replacing the pCI vector used in the construction of pGJA-P with pVAX1, the only vector authorized by the US Food and Drug Administration in clinical trials. Here, we report on our exploration of the kinetics of the antibody responses generated following pGJA-P/VAX immunization and the persistence of pGJA-P/VAX at both the inoculation site and the draining lymph nodes. Intranasal vaccination of mice with pGJA-P/VAX induced strong antibody responses that lasted for more than 6 months. Furthermore, pGJA-P/VAX could still be detected at both the inoculation site and the draining cervical lymph nodes 6 months after immunization. Thus, the persistent immune responses are likely due to the DNA depot in the host, which acts as a booster immunization.

译文

我们以前曾报道过,与非靶向抗龋DNA疫苗相比,靶向抗龋DNA疫苗pGJA-P诱导了加速和增加的抗体反应。最近,pGJA-P/VAX是一种用于人体试验的新型靶向抗龋齿DNA疫苗,通过用pVAX1代替用于构建pGJA-P的pCI载体,这是美国食品药品监督管理局在临床试验中唯一授权的载体。在这里,我们报告了我们对pGJA-P/VAX预防接种后产生的抗体反应的动力学以及pGJA-P/VAX在接种部位和引流淋巴结的持久性的探索。用pGJA-P/VAX对小鼠进行鼻内疫苗接种可诱导持续6个月以上的强烈抗体反应。此外预防接种后6个月,在接种部位和引流颈淋巴结中仍可检测到pGJA-P/VAX。因此,持续的免疫反应可能是由于宿主中的DNA储存库而起加强预防接种作用。

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