Tomato and pepper are widely grown in Oman for local consumption. A countrywide survey was conducted during 2010-2011 to collect samples and assess the diversity of begomoviruses associated with leaf curl disease of tomato and pepper. A virus previously only identified on the Indian subcontinent, chili leaf curl virus (ChLCV), was found associated with tomato and pepper diseases in all vegetable grown areas of Oman. Some of the infected plant samples were also found to contain a betasatellite. A total of 19 potentially full-length begomovirus and eight betasatellite clones were sequenced. The begomovirus clones showed >96% nucleotide sequence identity, showing them to represent a single species. Comparisons to sequences available in the databases showed the highest levels of nucleotide sequence identity (88.0-91.1%) to isolates of the "Pakistan" strain of ChLCV (ChLCV-PK), indicating the virus from Oman to be a distinct strain, for which the name Oman strain (ChLCV-OM) is proposed. An analysis for recombination showed ChLCV-OM likely to have originated by recombination between ChLCV-PK (the major parent), pepper leaf curl Lahore virus and a third strain of ChLCV. The betasatellite sequences obtained were shown to have high levels of identity to isolates of tomato leaf curl betasatellite (ToLCB) previous shown to be present in Oman. For the disease in tomato Koch's postulates were satisfied by Agrobacterium-mediated inoculation of virus and betasatellites clones. This showed the symptoms induced by the virus in the presence of the betasatellite to be enhanced, although viral DNA levels were not affected. ChLCV-OM is the fourth begomovirus identified in tomato in Oman and the first in Capsicum. The significance of these findings is discussed.

译文

:西红柿和胡椒在阿曼广泛种植供当地消费。在2010年至2011年期间,进行了一项全国性调查,以收集样本并评估与番茄和辣椒叶片卷曲病相关的初生病毒的多样性。在阿曼的所有蔬菜种植区,都发现了一种以前仅在印度次大陆上发现的病毒,即辣椒卷曲叶病毒(ChLCV),与番茄和辣椒病有关。还发现一些被感染的植物样品含有β卫星。总共对19个潜在的全长begomovirus和8个β卫星克隆进行了测序。该begomovirus克隆显示出> 96%的核苷酸序列同一性,表明它们代表单个物种。与数据库中可用序列的比较显示,与ChLCV的“巴基斯坦”毒株(ChLCV-PK)分离株的核苷酸序列同一性最高(88.0-91.1%),表明来自阿曼的病毒是不同的毒株建议使用阿曼菌株(ChLCV-OM)。重组分析显示,ChLCV-OM可能起源于ChLCV-PK(主要亲本),辣椒叶片卷曲拉合尔病毒和第三株ChLCV之间的重组。已显示获得的β卫星序列与先前证明存在于阿曼的番茄叶片卷曲β卫星(ToLCB)分离物具有高度同一性。对于番茄中的病害,农杆菌介导的病毒和小卫星克隆的接种使科赫的假设满意。这表明在β-卫星存在下由病毒诱导的症状得到了增强,尽管病毒DNA水平没有受到影响。 ChLCV-OM是在阿曼的番茄中鉴定出的第四种begomovirus,在辣椒中鉴定出了第一种。讨论了这些发现的意义。

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