The filamentous fungus Penicillium chrysogenum is used for the industrial production of β-lactam antibiotics. The pathway for β-lactam biosynthesis has been resolved and involves the enzyme phenylacetic acid CoA ligase that is responsible for the CoA activation of the side chain precursor phenylacetic acid (PAA) that is used for the biosynthesis of penicillin G. To identify ABC transporters related to β-lactam biosynthesis, we analyzed the expression of all 48 ABC transporters present in the genome of P. chryso-genum when grown in the presence and absence of PAA. ABC40 is significantly upregulated when cells are grown or exposed to high levels of PAA. Although deletion of this transporter did not affect β-lactam biosynthesis, it resulted in a significant increase in sensitivity to PAA and other weak acids. It is concluded that ABC40 is involved in weak acid detoxification in P. chrysogenum including resistance to phenylacetic acid.

译文

丝状真菌产黄青霉用于工业生产 β-内酰胺类抗生素。Β-内酰胺生物合成的途径已经解决,涉及苯乙酸CoA连接酶,该酶负责用于青霉素g生物合成的侧链前体苯乙酸 (PAA) 的CoA活化。为了鉴定与 β-内酰胺生物合成有关的ABC转运蛋白,我们分析了在存在和不存在PAA的情况下生长的chryso-genum基因组中存在的所有48个ABC转运蛋白的表达。当细胞生长或暴露于高水平的PAA时,ABC40显着上调。尽管该转运蛋白的缺失不会影响 β-内酰胺的生物合成,但会导致对PAA和其他弱酸的敏感性显着提高。结论是,ABC40参与了黄霉的弱酸解毒,包括对苯乙酸的抗性。

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