A strain of Pseudomonas aeruginosa isolated from a site contaminated with refined oil products exhibited demulsification capabilities against Tween 80-Span 80 stabilized oil-in-water (O/W), Tween 80-stabilized water-in-oil (W/O) model emulsions (kerosene-water), and an industrial emulsion (Daido Dairoll PA-5A). GC-MS analysis confirmed the presence of fatty acids and carbohydrates in the extracellular biodemulsifier. The demulsifying activity of cells and culture supernatants was favored by growth in media containing 1% diesel oil. There was a correlation between culture age, de-emulsification and cellular hydrophobicity, and highest activities were observed for cells and supernatants from 96-h cultures. Activity increased with addition of up to 60 mg cells or 300 μL supernatant to emulsions. The activity was relatively stable at 20-40 °C and to freezing, but was reduced by 69% by washing the cells with chloroform-methanol-water. This demulsifier has potential for application in biotreatment of emulsified oily wastewaters to promote recovery and/or degradation of oil.

译文

:从精制石油产品污染的地点分离出的铜绿假单胞菌菌株具有抗吐温80-跨度80稳定的水包油(O / W),吐温80稳定的油包水(W / O)的破乳能力模型乳液(煤油-水)和工业乳液(Daido Dairoll PA-5A)。 GC-MS分析证实了细胞外生物破乳剂中脂肪酸和碳水化合物的存在。在含有1%柴油的培养基中生长有利于细胞和培养上清液的破乳活性。在培养年龄,去乳化和细胞疏水性之间存在相关性,并且观察到来自96小时培养的细胞和上清液的最高活性。通过向乳剂中添加多达60 mg细胞或300μL上清液,活性得以提高。活性在20-40°C相对稳定,甚至可以冻结,但是通过用氯仿-甲醇-水洗涤细胞,活性降低了69%。该破乳剂有潜力用于乳化含油废水的生物处理,以促进油的回收和/或降解。

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