Carpesium macrocephalum (CM) Fr. et Sav. (Compositae) has been used in Chinese folk medicine as an analgesic, hemostatic, antipyretic, and to suppress inflammatory conditions. In the present study we aimed to provide scientific evidence for the anti-inflammatory properties of CM extract and evaluate the intrinsic mechanisms involved in both in vitro and in vivo experimental models. In in vitro findings, CM significantly inhibited the LPS-stimulated release of proinflammatory mediators such as nitric oxide, tumor necrosis factor-alpha, prostaglandin E2, and interleukin-6 in RAW264.7 macrophages in a concentration-dependent fashion. The attenuation of inflammatory responses in LPS-activated RAW264.7 cells by CM was closely associated with the suppression of nuclear factor-kappa B (NF-κB) phosphorylation, IκB-α degradation, and phosphorylation of Akt. CM treatment also attenuated the phosphorylation of STAT through TRIF dependent pathways in LPS-activated RAW264.7 cells. In vivo studies revealed that CM extract concentration dependently suppressed the acetic acid-induced vascular permeability in mice. Considering the data obtained regulation of multiple signaling mechanisms involving TRIF and Akt/NF-κB pathways might be responsible for the potent anti-inflammatory action of CM, substantiating its traditional use in inflammatory diseases.

译文

:大头翁(CM)Fr.等(菊科)在中国民间医学中已用作止痛,止血,解热和抑制炎性疾病的药物。在本研究中,我们旨在为CM提取物的抗炎特性提供科学依据,并评估涉及体内和体外实验模型的内在机制。在体外研究中,CM以浓度依赖性方式显着抑制RAW264.7巨噬细胞中LPS刺激的促炎性介质如一氧化氮,肿瘤坏死因子-α,前列腺素E2和白介素6的释放。 CM在LPS激活的RAW264.7细胞中减轻炎症反应与抑制核因子-κB(NF-κB)磷酸化,IκB-α降解和Akt磷酸化密切相关。 CM处理还通过LPS激活的RAW264.7细胞中的TRIF依赖性途径减弱了STAT的磷酸化。体内研究表明,CM提取物的浓度可依赖性地抑制乙酸诱导的小鼠血管通透性。考虑到所获得的数据,涉及TRIF和Akt /NF-κB途径的多种信号传导机制的调控可能是CM强大的抗炎作用,从而证实了其在炎性疾病中的传统用途。

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