CD28, described as a T cell costimulatory molecule so far, is expressed on human peripheral blood neutrophils, as shown by cell surface staining and immunoprecipitation with anti-CD28 monoclonal antibody, and by reverse transcription PCR. The phorbol 12-myristate 13-acetate-augmented expression of CD28 on these cells can be blocked by actinomycin D, an RNA transcription inhibitor, and staurosporin, a protein kinase inhibitor. Cross-linking of CD28 results in an early increase in IL-8 receptor A (IL-8RA or CXCR-1) expression and a concurrent increase in IL-8-induced chemotaxis. The expression of CXCR-1 is down-regulated by receptor internalization 3 h after CD28 cross-linking with concurrent decrease in IL-8-induced chemotactic migration. Thus, our results demonstrate for the first time that CD28 is expressed on human peripheral blood neutrophils and that CD28 may play an important role in the regulation of IL-8RA expression and migration of neutrophils in response to IL-8.

译文

:迄今为止,被描述为T细胞共刺激分子的CD28在人外周血中性粒细胞中表达,如细胞表面染色和用抗CD28单克隆抗体进行的免疫沉淀以及逆转录PCR所示。这些细胞上CD28的佛波12-肉豆蔻酸酯13-醋酸酯增强表达可以被放线菌素D(一种RNA转录抑制剂)和星形孢菌素(一种蛋白激酶抑制剂)阻断。 CD28的交联导致IL-8受体A(IL-8RA或CXCR-1)表达的早期增加,并同时引起IL-8诱导的趋化性增加。 CD28交联后3小时,受体内化作用下调了CXCR-1的表达,并同时降低了IL-8诱导的趋化性迁移。因此,我们的结果首次证明了CD28在人外周血中性粒细胞上表达,并且CD28可能在调节IL-8RA表达和中性粒细胞对IL-8的迁移中起重要作用。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录