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Decay of fluorescence polarization after an impulsive excitation is correlated with wobbling motion of fluorescent molecules in membranes. The motion is characterized by two parameters, a "wobbling diffusion constant" and a "degree of orientational constraint" both of which can be determined directly from experimentally obtained decay. Detailed discussion, including theoretically calculated time-courses of polarization decay, is given for several types of molecules embedded in lipid bilayers; these types cover a large part of fluorescent probes available at present. The theory is useful for the analysis of fluorescence polarization decay in any system where the orientation of fluorophore is restricted by the surrounding structure.

译文

脉冲激发后荧光偏振的衰减与膜中荧光分子的摆动运动有关。该运动的特征在于两个参数,“波动扩散常数”和“取向约束度”,这两个参数都可以直接从实验获得的衰减中确定。对嵌入脂质双分子层中的几种类型的分子进行了详细的讨论,包括理论上计算的极化衰减时间过程。这些类型涵盖了目前可用的大部分荧光探针。该理论对于分析荧光团的方向受周围结构限制的任何系统中的荧光偏振衰减非常有用。

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