Only a few methods permit researchers to study selected regions of the central and peripheral nervous systems with a spatial and time resolution sufficient to image the function of neural structures. Usually, these methods cannot analyse deep-brain regions and a high-resolution method, which could repeatedly probe dynamic processes in any region of the central and peripheral nervous systems, is much needed. Here, we show that fibred fluorescence microscopy-which uses a small-diameter fibre-optic probe to provide real-time images-has the spatial resolution to image various neural structures in the living animal, the consistency needed for a sequential, quantitative evaluation of axonal degeneration/regeneration of a peripheral nerve, and the sensitivity to detect calcium transients on a sub-second timescale. These unique features should prove useful in many physiological studies requiring the in situ functional imaging of tissues in a living animal.

译文

:只有几种方法可以使研究人员以足够的空间和时间分辨率来研究中枢神经系统和周围神经系统的选定区域,以对神经结构的功能进行成像。通常,这些方法无法分析深脑区域,因此非常需要高分辨率的方法,该方法可以反复探查中枢神经系统和周围神经系统任何区域的动态过程。在这里,我们显示纤维荧光显微镜法(使用小直径光纤探针提供实时图像)具有空间分辨率,可以对活体动物的各种神经结构进行成像,具有连续定量地评估动物所需的一致性。周围神经的轴突变性/再生,以及在亚秒级范围内检测钙瞬变的敏感性。这些独特的特征在许多需要活体动物组织原位功能成像的生理研究中应被证明是有用的。

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