BACKGROUND/AIMS:The hepatitis B virus (HBV) infection course is divided into 4 immune phases which were mainly characterized by clinical markers. We investigated the immune markers, especially inhibitory receptors, cytokine and chemokine expressions among the immune phases especially between immune tolerance (IT) phase and immune control (IC) phase. METHODOLOGY:Blood and serum samples of 64 patients and serum samples of 22 healthy controls were obtained. We used flow cytometric methods for measurements of PD-1, PD-L1 and flow fluorescence immunoassay for the serum cytokines and chemokines concentrations. IL-27 was measured by ELISA and the receptor IL-27R was detected too. RESULTS:The proportions of PD1 positive cells in CD4+, CD4+CD45RO+, and CD8+ T-cell subsets in IC phase were greater than in IC phase. The frequencies of PD1 expressions in CD8+pentamer+ and CD8+CD45RA-pentamer+ T cells were higher in IC phase than in IT and ICC phases. The serum concentration of IL-27 in IT group was higher than in IC, ICC and HC groups. Concentrations of cytokines TNF-α and IL-10 and chemokines RANTES, IL-8 and IP-10 were higher in HBV infected patients. CONCLUSIONS:The reduction of percentages of PD-1 positive cells may contribute to estimate entering the IC phase and decide the opportune moment to start antivirus therapy.

译文

背景/目的:乙肝病毒(HBV)感染过程分为四个免疫阶段,主要以临床标志物为特征。我们调查了免疫阶段,尤其是免疫耐受(IT)阶段和免疫控制(IC)阶段之间的免疫标志物,特别是抑制性受体,细胞因子和趋化因子的表达。
方法:获得64例患者的血液和血清样本以及22例健康对照者的血清样本。我们使用流式细胞仪方法测量PD-1,PD-L1,并使用流式荧光免疫法测定血清细胞因子和趋化因子的浓度。通过ELISA测量IL-27,并且也检测到受体IL-27R。
结果:IC期CD4,CD4,CD45RO和CD8 T细胞亚群中PD1阳性细胞的比例大于IC期。 CD8五聚体和CD8 CD45RA-五聚体T细胞中PD1表达的频率在IC期高于IT和ICC期。 IT组的血清IL-27浓度高于IC,ICC和HC组。 HBV感染患者的细胞因子TNF-α和IL-10以及趋化因子RANTES,IL-8和IP-10的浓度较高。
结论:PD-1阳性细胞百分比的降低可能有助于估计进入IC阶段并决定开始抗病毒治疗的时机。

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