Construction of tissue microarrays (TMAs) to efficiently characterize large sets of noninvasive epithelial lesions in the breast by immunohistochemistry is an appealing investigative approach, but presents technical challenges. We report methodologic studies performed to optimize methods for building TMAs from noninvasive breast tissues collected in a large case-control study of breast cancer. Using a manual arraying technique with 2.0-mm diameter needles, we constructed TMAs from specimens obtained from 32 women with breast cancer containing the following targets: (1) 28 terminal duct lobular units (TDLUs); (2) 28 ductal carcinomas in situ, and (3) 23 invasive carcinomas. Using careful target selection, we achieved representation of approximately 80% of noninvasive targets with sustained preservation through section 30 of the TMAs. Immunohistochemical staining of TDLU targets demonstrated positive staining for estrogen receptor (ER) in 30.8% of tubules and for progesterone receptor (PR) in 50.0%. To establish an efficient method to evaluate staining results in TDLUs, we created a categorical scoring system to approximate the percentage of tubules containing positive stained cells (<10%, 10% to 50%, >or=50%), and compared the results with those obtained by tubule counting. Comparison between the two methods demonstrated exact agreement for 70.8% of ER and 79.2% of PR stains without two-category discrepancies. ER/PR expression levels in multiple (up to 4) noninvasive targets of the same tissue type (TDLU or DCIS) from a single block showed good correlation. These data suggest that it is feasible to produce TMAs of noninvasive breast structures, albeit with careful selection of targets, and that immunostains of such cores may permit efficient immunohistochemical characterization of peritumoral tissues. Additional exploration of this approach is needed.

译文

:通过免疫组织化学法构建组织微阵列(TMA)以有效表征乳腺中大量非侵袭性上皮病变的方法是一种有吸引力的研究方法,但也带来了技术挑战。我们报告进行的方法学研究,以优化从大型乳腺癌病例对照研究中收集的无创乳腺组织中构建TMA的方法。我们使用直径为2.0毫米针头的手动排列技术,从32位乳腺癌女性的标本中构建了TMA,这些标本包括以下目标:(1)28个末梢小叶单位(TDLU); (2)28例原位导管癌,(3)23例浸润性癌。通过精心选择目标,我们通过TMA第30节实现了约80%的非侵入性目标的代表,并得到了持续保存。 TDLU靶标的免疫组织化学染色显示,在30.8%的肾小管中,雌激素受体(ER)阳性染色,在50.0%中,孕激素受体(PR)染色阳性。为了建立一种有效的方法来评估TDLU中的染色结果,我们创建了一个分类评分系统,以估计含有阳性染色细胞的小管的百分比(<10%,10%至5​​0%,>或= 50%),并比较结果与那些通过肾小管计数获得的。两种方法之间的比较表明,ER染色的70.8%和PR染色的79.2%完全吻合,没有两类差异。单个区域中相同组织类型(TDLU或DCIS)的多个(多达4个)非侵入性靶标中的ER / PR表达水平显示出良好的相关性。这些数据表明,尽管精心选择了靶标,但生产无创性乳房结构的TMA是可行的,并且此类核心的免疫染色可允许对肿瘤周围组织进行有效的免疫组织化学表征。需要对该方法进行其他探索。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录