Molecular biology techniques are applied for the diagnosis of meningoencephalitis due to herpesviruses, enteroviruses or polyomaviruses, for the diagnosis of human cytomegalovirus, human parvovirus B19, varicella-zoster virus and rubella virus infections occurring during pregnancy, for the diagnosis and the management of retrovirus infections (HIV and HTLV) and of hepatitis (HBV and HCV), for papillomavirus typing and to detect a link between virus and clinical manifestations (cardiomyopathy or insulinodependent diabetes with coxsackievirus B: Kaposi's sarcoma with HHV 8) or to investigate an environmental contamination with viruses. These new molecular markers which are both qualitative and quantitative represent an important advance in the field of viral diagnosis research, in the monitoring of viral load during the course of infection, in the therapy control of viral disease and in the epidemiology of virus spread. Standardization and automatization are obtained using available commercial reagents and kits.

译文

:分子生物学技术被用于诊断由疱疹病毒,肠病毒或多瘤病毒引起的脑膜脑炎,用于诊断人巨细胞病毒,人细小病毒B19,水痘-带状疱疹病毒和风疹病毒在怀孕期间发生的感染,用于诊断和管理逆转录病毒感染(HIV和HTLV)和肝炎(H​​BV和HCV),用于乳头瘤病毒分型并检测病毒与临床表现之间的联系(心肌病或胰岛素依赖型糖尿病与柯萨奇病毒B:卡波西氏肉瘤伴HHV 8)或调查环境污染病毒。这些新的定性和定量分子标记代表了病毒诊断研究领域,感染过程中病毒载量的监测,病毒疾病的治疗控制和病毒传播的流行病学的重要进展。使用可用的市售试剂和试剂盒可获得标准化和自动化。

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