Research on multiple sclerosis (MS) frequently requires typing for allele HLA-DRB1*1501, which the complexities of the HLA system can restrict to specialised histocompatibility laboratories. To overcome this limitation, we have implemented a simple, robust and highly specific method for DRB1*1501 detection. One single-tube polymerase-chain reaction (PCR) per DNA sample allows for detecting DR2 individuals. The spare PCR products of these are then sequenced to identify allele DRB1*1501 by comparison with the official, publicly accessible HLA database. This approach, much simpler than previously available methods, should facilitate research on MS by making accurate identification of DRB1*1501 accessible to neuroscience laboratories.

译文

:对多发性硬化症(MS)的研究通常需要输入等位基因HLA-DRB1 * 1501,HLA系统的复杂性可能会限制于专门的组织相容性实验室。为克服此限制,我们为DRB1 * 1501检测实现了一种简单,可靠且高度特定的方法。每个DNA样品一个单管聚合酶链反应(PCR)即可检测DR2个体。然后,通过与官方的可公开访问的HLA数据库进行比较,对这些样品的备用PCR产物进行测序,以鉴定等位基因DRB1 * 1501。这种方法比以前可用的方法简单得多,应该通过使神经科学实验室可以准确识别DRB1 * 1501来促进对MS的研究。

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