Germinal centers (GCs) support high-affinity, long-lived humoral immunity. How memory B cells develop in GCs is not clear. Through the use of a cell-cycle-reporting system, we identified GC-derived memory precursor cells (GC-MP cells) that had quit cycling and reached G0 phase while in the GC, exhibited memory-associated phenotypes with signs of affinity maturation and localized toward the GC border. After being transferred into adoptive hosts, GC-MP cells reconstituted a secondary response like genuine memory B cells. GC-MP cells expressed the interleukin 9 (IL-9) receptor and responded to IL-9. Acute treatment with IL-9 or antibody to IL-9 accelerated or retarded the positioning of GC-MP cells toward the GC edge and exit from the GC, and enhanced or inhibited the development of memory B cells, which required B cell-intrinsic responsiveness to IL-9. Follicular helper T cells (TFH cells) produced IL-9, and deletion of IL-9 from T cells or, more specifically, from GC TFH cells led to impaired memory formation of B cells. Therefore, the GC development of memory B cells is promoted by TFH cell-derived IL-9.

译文

生发中心 (GCs) 支持高亲和力、长寿的体液免疫。记忆b细胞如何在GCs中发育尚不清楚。通过使用细胞周期报告系统,我们确定了GC衍生的记忆前体细胞 (gc-mp细胞),这些细胞在GC中退出循环并达到G0期,表现出与记忆相关的表型,并具有亲和成熟的迹象,并定位于GC边界。转移到过继宿主后,gc-mp细胞像真正的记忆b细胞一样重建了次级反应。Gc-mp细胞表达白介素9 (IL-9) 受体并对IL-9产生反应。用IL-9或抗体IL-9的急性治疗加速或延迟了gc-mp细胞朝向GC边缘的定位并从GC退出,并增强或抑制了记忆b细胞的发育,这需要b细胞对IL-9的内在反应性。滤泡辅助性T细胞 (TFH细胞) 产生IL-9,T细胞或更具体地说,GC TFH细胞中IL-9的缺失导致b细胞的记忆形成受损。因此,TFH细胞衍生的IL-9促进了记忆b细胞的GC发育。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录