Technologies to assess the molecular targets of biomolecules in living cells are lacking. We have developed a technology called magnetism-based interaction capture (MAGIC) that identifies molecular targets on the basis of induced movement of superparamagnetic nanoparticles inside living cells. Efficient intracellular uptake of superparamagnetic nanoparticles (coated with a small molecule of interest) was mediated by a transducible fusogenic peptide. These nanoprobes captured the small molecule's labeled target protein and were translocated in a direction specified by the magnetic field. Use of MAGIC in genome-wide expression screening identified multiple protein targets of a drug. MAGIC was also used to monitor signal-dependent modification and multiple interactions of proteins.

译文

缺乏评估活细胞中生物分子分子靶标的技术。我们开发了一种称为基于磁性的相互作用捕获 (MAGIC) 的技术,该技术可根据活细胞内超顺磁性纳米颗粒的诱导运动来识别分子靶标。超顺磁性纳米颗粒 (涂有目标小分子) 的有效细胞内摄取是由可传递的融合肽介导的。这些纳米探针捕获了小分子标记的靶蛋白,并在磁场指定的方向上易位。在全基因组表达筛选中使用MAGIC确定了药物的多个蛋白质靶标。MAGIC还用于监测信号依赖性修饰和蛋白质的多种相互作用。

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