Human apurinic/apyrimidinic endonuclease/redox effector factor 1 (APE1) is an essential DNA repair protein. Herein, we demonstrate that avidin-oriented abasic site-containing DNA strands (AP-DNA) on the surface of silica coated magnetic nanoparticles (SiMNP) can selectively respond to APE1 while resist the digestion by other nucleases. Mechanism studies have revealed that avidin may serve as an organizer protein and recruit APE1 to the DNA substrates on the nanoparticles via strong and specific interactions. Taking advantage of this newly disclosed property, we for the first time successfully displayed the intracellular activities of APE1 in living cells by fluorescence imaging. The avidin organized AP-DNA-SiMNP assembly holds great potential for enzyme-mediated release of drugs inside tumor cells which often contain higher levels of APE1 than normal cells.

译文

人嘌呤/嘧啶核酸内切酶/氧化还原效应因子1 (APE1) 是一种必需的DNA修复蛋白。在本文中,我们证明了二氧化硅涂覆的磁性纳米颗粒 (SiMNP) 表面上的面向抗生物素蛋白的含碱基位点的DNA链 (ap-dna) 可以选择性地响应APE1,同时抵抗其他核酸酶的消化。机理研究表明,抗生物素蛋白可以作为组织蛋白,并通过强而特异性的相互作用将APE1募集到纳米颗粒上的DNA底物中。利用这种新发现的特性,我们首次通过荧光成像成功地显示了活细胞中APE1的细胞内活性。抗生物素蛋白组织的AP-DNA-SiMNP组装具有酶介导的药物在肿瘤细胞内释放的巨大潜力,而肿瘤细胞通常含有比正常细胞更高水平的APE1。

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