Biological synthesis of nanoparticles is a growing research trend because it has numerous applications in pharmaceutics and biomedicine. The aim of this study was to obtain silver nanoparticles (AgNPs) from Bacillus sp. KFU36, a marine strain, and to assess its anticancer activity. The supernatant of Bacillus sp. KFU36 was supplemented with silver nitrate and the nanoparticles obtained were characterized spectrophotometrically and microscopically. A band of surface plasmon resonance was appeared at 430 nm, as revealed by UV-vis spectrophotometry. X-ray diffraction spectrum and Energy Dispersive Spectroscopy confirmed the crystalline and metallic structure of the AgNPs, respectively. Scanning electron microscopy revealed that the shape of the synthesized AgNPs were spherical and the size extended between 5 and 15 nm. The AgNPs were investigated for their potential anticancer effects on the cell viability, migration and apoptosis using MTT and wound-healing assays, and flow cytometry, respectively. The cytotoxic effects of these nanoparticles were evidenced by the decreasing the cell viability (as 15% at 50 μg/ml), cell density, adhesion capacity and losing the normal shape and size, and inducing the apoptosis on MCF-7 by 61% at 50 μg/ml. These findings confirm that the synthesized AgNPs exhibited superior anticancer activities and therefore could be exploited as a promising, cost-effective, and environmentally benign strategy in treating this disease in future.

译文

纳米颗粒的生物合成是一个日益发展的研究趋势,因为它在药剂学和生物医学中有许多应用。这项研究的目的是从海洋芽孢杆菌属KFU36中获得银纳米颗粒 (AgNPs),并评估其抗癌活性。在芽孢杆菌属的上清液中添加了硝酸银,并通过分光光度法和显微镜对获得的纳米颗粒进行了表征。如通过UV-vis分光光度法所揭示的,在430 nm处出现了表面等离子体共振带。X射线衍射光谱和能量色散光谱分别证实了AgNPs的晶体和金属结构。扫描电子显微镜显示,合成的agnp的形状为球形,尺寸在5至15 nm之间扩展。分别使用MTT和伤口愈合试验以及流式细胞术研究了AgNPs对细胞活力,迁移和凋亡的潜在抗癌作用。这些纳米颗粒的细胞毒性作用通过降低细胞活力 (以50 μ g/ml 15%) 、细胞密度、粘附能力和失去正常形状和大小,以及以50 μ g/ml 61% 诱导MCF-7凋亡来证明。这些发现证实了合成的AgNPs具有出色的抗癌活性,因此可以作为将来治疗该疾病的有前途,具有成本效益且对环境无害的策略。

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