Nanomaterials hold great promise in the manipulation and treatments of mesenchymal stem cells, since they allow the modulation of their properties and differentiation. However, systematic studies have to be carried out in order to assess their potential toxicological effects. The present study reports on biocompatibility evaluation of glycol-chitosan coated barium titanate nanoparticles (BTNPs) on rat mesenchymal stem cells (MSCs). BTNPs are a class of ceramic systems which possess interesting features for biological applications thanks to their peculiar dielectric and piezoelectric properties. Viability was evaluated up to 5 days of incubation (concentrations in the range 0-100 μg/ml) both quantitatively and qualitatively with specific assays. Interactions cells/nanoparticles were further investigated with analysis of the cytoskeleton conformation, with SEM and TEM imaging, and with AFM analysis. Finally, differentiation in adipocytes and osteocytes was achieved in the presence of high doses of BTNPs, thus highlighting the safety of these nanostructures towards mesenchymal stem cells.

译文

纳米材料在间充质干细胞的操作和治疗中具有广阔的前景,因为它们可以调节其特性和分化。但是,必须进行系统的研究以评估其潜在的毒理学作用。本研究报道了乙二醇-壳聚糖包被的钛酸钡纳米颗粒 (BTNPs) 对大鼠间充质干细胞 (MSCs) 的生物相容性评价。BTNPs是一类陶瓷系统,由于其独特的介电和压电特性,具有用于生物应用的有趣功能。通过特定测定定量和定性地评估孵育5天 (浓度在0-100 μ g/ml范围内) 的活力。通过分析细胞骨架构象,SEM和TEM成像以及AFM分析进一步研究了细胞/纳米颗粒的相互作用。最后,在高剂量BTNPs的存在下实现了脂肪细胞和骨细胞的分化,从而突出了这些纳米结构对间充质干细胞的安全性。

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