We tried to evaluate the possible involvement of fetuin in the scavenger receptors (SRs)-mediated hepatic uptake of polystyrene nanospheres with the size of 50 nm (NS-50), which has surface negative charge (zeta potential=-21.8+/-2.3 mV). The liver perfusion studies in rats revealed that the hepatic uptake of NS-50 pre-coated with fetuin (NS-50-fetuin) was significantly inhibited by poly inosinic acid (poly I), a typical inhibitor of SRs, whereas that of plain NS-50 or NS-50 pre-coated with BSA (NS-50-BSA) was not. The uptake of NS-50-fetuin by cultured Kupffer cells was also significantly inhibited by poly I, and anti-class A scavenger receptors (SR-A) antibody, suggesting that fetuin on NS-50 mediated the recognition and internalization of NS-50 by Kupffer cells and at least SR-A would be responsible for the uptake. Taken that Western blot analysis confirmed that fetuin certainly adsorbed on the surface of NS-50 after the incubation of NS-50 with serum, the results obtained in the present study indicate that fetuin would be one of the serum proteins that were substantially involved in the hepatic uptake of NS-50 via SRs.

译文

我们试图评估胎球蛋白可能参与清道夫受体 (SRs) 介导的聚苯乙烯纳米球的肝摄取,其尺寸为50 nm (NS-50),具有表面负电荷 (ζ 电位 =-21.8/-2.3 mV)。大鼠的肝脏灌注研究表明,预包被胎球蛋白 (NS-50-fetuin) 的NS-50的肝脏摄取被典型的SRs抑制剂聚肌苷酸 (poly I) 显着抑制,而普通NS-50或预包被的NS-50的肝脏摄取BSA (NS-50-BSA) 没有。培养的库普弗细胞对NS-50-fetuin的摄取也被poly I和抗A类清道夫受体 (sr-a) 抗体显着抑制,这表明NS-50上的胎球蛋白介导了库普弗细胞对NS-50的识别和内化,至少sr-a将负责摄取。认为Western印迹分析证实胎球蛋白在将NS-50与血清孵育后肯定吸附在NS-50表面,本研究获得的结果表明,胎球蛋白将是基本上参与肝脏摄取的血清蛋白之一NS-50通过SRs。

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